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    pgp95-antibody-epr4118-ab108986.pdf

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Neuroscience Neurology process Neurodegenerative disease Parkinson's disease Parkin / PARK
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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-PGP9.5 抗体 [EPR4118] (ab108986)

  • Datasheet
  • SDS
Reviews (11)Q&A (1)References (43)

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Western blot - Anti-PGP9.5 antibody [EPR4118] (ab108986)
  • Western blot - Anti-PGP9.5 antibody [EPR4118] (ab108986)
  • Immunohistochemistry (Frozen sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)
  • Immunocytochemistry/ Immunofluorescence - Anti-PGP9.5 antibody [EPR4118] (ab108986)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)
  • Flow Cytometry (Intracellular) - Anti-PGP9.5 antibody [EPR4118] (ab108986)
  • Immunoprecipitation - Anti-PGP9.5 antibody [EPR4118] (ab108986)
  • Western blot - Anti-PGP9.5 antibody [EPR4118] (ab108986)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)
  • Flow Cytometry (Intracellular) - Anti-PGP9.5 antibody [EPR4118] (ab108986)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)
  • Western blot - Anti-PGP9.5 antibody [EPR4118] (ab108986)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)
  • Anti-PGP9.5 antibody [EPR4118] (ab108986)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4118] to PGP9.5 - Neuronal Marker
  • Suitable for: ICC/IF, Flow Cyt (Intra), IHC-Fr, WB, IP, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 647 Carrier Free

こちらの製品もご検討ください

一次抗体
Product image
Anti-Calcitonin antibody [SP17] (ab16697)
一次抗体
Product image
Alexa Fluor® 647 Anti-PGP9.5 antibody [EPR4118] (ab196173)
一次抗体
Product image
Anti-PGP9.5 antibody [EPR4118] - BSA and Azide free (ab220823)

関連製品

製品の概要

  • 製品名

    Anti-PGP9.5 antibody [EPR4118]
    PGP9.5 一次抗体 製品一覧
  • 製品の詳細

    Rabbit monoclonal [EPR4118] to PGP9.5 - Neuronal Marker
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: ICC/IF, Flow Cyt (Intra), IHC-Fr, WB, IP, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ・コントロール

    • WB: Fetal brain, Y79, U87-MG, SH-SY5Y, HAP1, HeLa and HEK-293T cell lysates; IHC-P: Human glioma, colon, and hepatocellular carcinoma tissue, mouse colon, mouse cerebral cortex tissue, rat Jejunum and cerebral cortex tissue; ICC/IF: Neuro-2a cells; IP: Human fetal brain lysate; Flow Cyt (intra): SH-SY5Y and Y79 cells; IHC-Fr: Mouse cerebrum tissue.
  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    EPR4118
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Parkin / PARK
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Deubiquitination
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Neuronal
    • Neuroscience
    • Diseases

関連製品

  • Alternative Versions

    • Alexa Fluor® 647 Anti-PGP9.5 antibody [EPR4118] (ab196173)
    • Anti-PGP9.5 antibody [EPR4118] - BSA and Azide free (ab220823)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human UCHL1 (PGP9.5) knockout HEK-293T cell line (ab255443)
  • KO cell lysates

    • Human UCHL1 (PGP9.5) knockout HEK-293T cell lysate (ab263773)
  • KO cell pellets

    • Human UCHL1 (PGP9.5) knockout HEK-293T cell pellet (ab278928)
  • Recombinant Protein

    • Recombinant human PGP9.5 protein (ab53962)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab108986の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF (1)
1/500.
Flow Cyt (Intra)
1/100 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-Fr
1/250.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

WB
1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).
IP
1/10 - 1/100.
IHC-P (10)
1/250 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
ICC/IF
1/500.
Flow Cyt (Intra)
1/100 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-Fr
1/250.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

WB
1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).
IP
1/10 - 1/100.
IHC-P
1/250 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
  • 組織特異性

    Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
  • 関連疾患

    Parkinson disease 5
    Neurodegeneration with optic atrophy, childhood-onset
  • 配列類似性

    Belongs to the peptidase C12 family.
  • 翻訳後修飾

    O-glycosylated.
  • 細胞内局在

    Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
  • Target information above from: UniProt accession P09936 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 7345 Human
    • Entrez Gene: 22223 Mouse
    • Entrez Gene: 29545 Rat
    • Omim: 191342 Human
    • SwissProt: P09936 Human
    • SwissProt: Q9R0P9 Mouse
    • SwissProt: Q00981 Rat
    • Unigene: 518731 Human
    • Unigene: 29807 Mouse
    • Unigene: 107213 Rat
    see all
  • 別名

    • Epididymis luminal protein 117 antibody
    • Epididymis secretory protein Li 53 antibody
    • HEL 117 antibody
    • HEL S 53 antibody
    • NDGOA antibody
    • Neuron cytoplasmic protein 9.5 antibody
    • OTTHUMP00000218137 antibody
    • OTTHUMP00000218139 antibody
    • OTTHUMP00000218140 antibody
    • OTTHUMP00000218141 antibody
    • Park 5 antibody
    • PARK5 antibody
    • PGP 9.5 antibody
    • PGP9.5 antibody
    • PGP95 antibody
    • Protein gene product 9.5 antibody
    • Ubiquitin C terminal esterase L1 antibody
    • Ubiquitin C terminal hydrolase antibody
    • Ubiquitin C terminal hydrolase L1 antibody
    • Ubiquitin carboxyl terminal esterase L1 antibody
    • Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody
    • Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody
    • Ubiquitin thioesterase L1 antibody
    • Ubiquitin thiolesterase antibody
    • Ubiquitin thiolesterase L1 antibody
    • UCH-L1 antibody
    • UCHL1 antibody
    • UCHL1_HUMAN antibody
    see all

画像

  • Western blot - Anti-PGP9.5 antibody [EPR4118] (ab108986)
    Western blot - Anti-PGP9.5 antibody [EPR4118] (ab108986)
    All lanes : Anti-PGP9.5 antibody [EPR4118] (ab108986) at 1/1000 dilution

    Lane 1 : Wild-type Hap1 cell lysate
    Lane 2 : UCHL1 knockout Hap1 cell lysate
    Lane 3 : SH-SY5Y cell lysate
    Lane 4 : Wild-type HEK-293T cell lysate
    Lane 5 : UCHL1 knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 24 kDa



    Lanes 1 - 5: Merged signal (red and green). Green - ab108986 observed at 25 kDa. Red - loading control, ab8245 observed at 37 kDa.

    ab108986 was shown to react with PGP9.5 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255443 (knockout cell lysate ab263773) was used. Wild-type and PGP9.5 knockout samples were subjected to SDS-PAGE. ab108986 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PGP9.5 antibody [EPR4118] (ab108986)
    Western blot - Anti-PGP9.5 antibody [EPR4118] (ab108986)
    All lanes : Anti-PGP9.5 antibody [EPR4118] (ab108986) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : UCHL1 knockout HAP1 whole cell lysate
    Lane 3 : SH-SY5Y whole cell lysate
    Lane 4 : HEK293 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 24 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab108986 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

    Ab108986 was shown to specifically react with UCHL1 in wild-type cells as signal was lost in UCHL1 knockout HAP1 cells. Wild-type and UCHL1 knockout samples were subjected to SDS-PAGE.  Ab108986 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Frozen sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)
    Immunohistochemistry (Frozen sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)

    Immunohistochemistry (Frozen sections) analysis of mouse cerebrum tissue sections labeling PGP9.5 with Purified ab108986 at 1/250 (0.5 µg/ml).Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

  • Immunocytochemistry/ Immunofluorescence - Anti-PGP9.5 antibody [EPR4118] (ab108986)
    Immunocytochemistry/ Immunofluorescence - Anti-PGP9.5 antibody [EPR4118] (ab108986)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cell line) cells labeling PGP9.5 with ab108986 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Neuro-2a cell line. The nuclear counter stain is DAPI (blue).

    The negative control is PBS only.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)

    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling PGP9.5 with ab108986, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on human hepatocellular carcinoma. The section was incubated with ab229902 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

  • Flow Cytometry (Intracellular) - Anti-PGP9.5 antibody [EPR4118] (ab108986)
    Flow Cytometry (Intracellular) - Anti-PGP9.5 antibody [EPR4118] (ab108986)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Y79 (Human retinoblastoma retinoblastoma)cells labelling PGP9.5 with ab108986 at 1/20 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (black)and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

     

     

  • Immunoprecipitation - Anti-PGP9.5 antibody [EPR4118] (ab108986)
    Immunoprecipitation - Anti-PGP9.5 antibody [EPR4118] (ab108986)

    PGP9.5 was immunoprecipitated from 0.35 mg Human fetal brain lysate  with ab108986 at 1/20 dilution (0.5μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab108986 1/500 dilution (0.17 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/1000 dilution.

    Lane 1: Human fetal brain lysate 10μg
    Lane 2: ab108986 IP in Human fetal brain lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab108986 in Human fetal brain lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

  • Western blot - Anti-PGP9.5 antibody [EPR4118] (ab108986)
    Western blot - Anti-PGP9.5 antibody [EPR4118] (ab108986)
    All lanes : Anti-PGP9.5 antibody [EPR4118] (ab108986) at 1/5000 dilution

    Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates
    Lane 3 : C6 (Rat glial tumor glial cell) whole cell lysates
    Lanes 4 & 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 5 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates
    Lane 7 : Mouse brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 24 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)

    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling PGP9.5 with ab108986, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on rat cerebral cortex.The section was incubated with ab229902 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)

    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling PGP9.5 with ab108986, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on mouse cerebral cortex. The section was incubated with ab229902 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)This image is courtesy of an annonymous Abreview

    Formalin-fixed, paraffin-embedded Cat lung tissue stained for PGP9.5 using ab108986 at 1/250 dilution in immunohistochemical analysis. The secondary antibody was ImmPRESS™ HRP Universal Antibody (Anti-Mouse IgG/A). Antigen retrieval: Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0.

    See Abreview

  • Flow Cytometry (Intracellular) - Anti-PGP9.5 antibody [EPR4118] (ab108986)
    Flow Cytometry (Intracellular) - Anti-PGP9.5 antibody [EPR4118] (ab108986)

    Overlay histogram showing SH-SY5Y cells stained with ab108986 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108986, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluorr® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. 

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)This image is courtesy of an Abreview submitted by Carl Hobbs

    Immunohistochemical analysis of rat Jejunum tissue sections labeling PGP9.5 with ab108986 at a dulution of 1/1000. Sections were fixed with Formaldehyde. A Biotin conjugated Goat Anti-Rabbit IgG at 1/300 was used as the secondary antibody. Antigen retrieval was heat mediated using citric acid. 

    All nerve components of enteric plexuses appear to be very well demonstrated, particularly the fine fibres of the lamina propria and the muscularis mucosa.

    See Abreview

  • Western blot - Anti-PGP9.5 antibody [EPR4118] (ab108986)
    Western blot - Anti-PGP9.5 antibody [EPR4118] (ab108986)
    All lanes : Anti-PGP9.5 antibody [EPR4118] (ab108986) at 1/1000 dilution

    Lane 1 : Fetal brain cell lysate
    Lane 2 : Y79 cell lysate
    Lane 3 : U87-MG cell lysate
    Lane 4 : SH-SY5Y cell lysate
    Lane 5 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 24 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)This image was courtesy of an annonymous Abreview

    Fomalin-fixed, paraffin-embedded Dog skin tissue stained for PGP9.5 using ab108986 at 1/500 dilution in immunohistochemical analysis. ImmPRESS™ Anti-Rabbit IgG Polymer Detection Kit was used as the secondary antibody.

    Antigen Retrieval: Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)This image is courtesy of an Abreview submitted by Carl Hobbs.

    ab108986 staining PGP9.5 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with the primary antibody (1/500 in TBS/BSA/azide) for 16 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)This image is courtesy of an Abreview submitted by Carl Hobbs

    Immunohistochemical analysis of mouse colon tissue sections labeling PGP9.5 with ab108986 at a dulution of 1/1500. Sections were fixed with Formaldehyde. A Biotin conjugated Goat Anti-Rabbit IgG at 1/300 was used as the secondary antibody. Antigen retrieval was heat mediated using citric acid. 

    All nerve cell/fibre components of enteric plexuses are demonstrated very well.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4118] (ab108986)
    Immunohistochemical staining of PGP9.5 in paraffin embedded Human glioma tissue, using ab108986 at a 1/250 dilution.
  • Anti-PGP9.5 antibody [EPR4118] (ab108986)
    Anti-PGP9.5 antibody [EPR4118] (ab108986)

プロトコール

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (43)

ab108986 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab108986 は 43 報の論文で使用されています。

  • Zhang PF  et al. Integrated analysis of phosphoproteome and ubiquitylome in epididymal sperm of buffalo (Bubalus bubalis). Mol Reprod Dev 88:15-33 (2021). PubMed: 33140506
  • Chen SJ  et al. Targeting lysosomal cysteine protease cathepsin S reveals immunomodulatory therapeutic strategy for oxaliplatin-induced peripheral neuropathy. Theranostics 11:4672-4687 (2021). PubMed: 33754020
  • Hori Y  et al. Cardiac afferent signaling partially underlies premature ventricular contraction-induced cardiomyopathy. Heart Rhythm N/A:N/A (2021). PubMed: 33845214
  • Campbell-Thompson M & Tang SC Pancreas Optical Clearing and 3-D Microscopy in Health and Diabetes. Front Endocrinol (Lausanne) 12:644826 (2021). PubMed: 33981285
  • Yuan PQ  et al. Intrinsic cholinergic innervation in the human sigmoid colon revealed using CLARITY, three-dimensional (3D) imaging, and a novel anti-human peripheral choline acetyltransferase (hpChAT) antiserum. Neurogastroenterol Motil 33:e14030 (2021). PubMed: 33174295
View all Publications for this product

レビューと Q&A

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1-10 of 12 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-PGP9.5 antibody [EPR4118]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hPSC-derived neurons)
Permeabilization
Yes - 0.5% Triton + 0.1% BSA
Specification
hPSC-derived neurons
Blocking step
0.1% BSA/ 10% FCS as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde
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投稿 Jun 17 2022

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PGP9.5 antibody [EPR4118]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dolphin Tissue sections (Clitoris)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M Citrate Buffer
Permeabilization
No
Specification
Clitoris
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Read More
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投稿 Jul 06 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PGP9.5 antibody [EPR4118]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Read More
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投稿 Jul 19 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PGP9.5 antibody [EPR4118]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
liver
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Jul 17 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PGP9.5 antibody [EPR4118]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Finch Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Jun 24 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PGP9.5 antibody [EPR4118]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
placenta
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 May 20 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PGP9.5 antibody [EPR4118]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (foot pad)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
foot pad
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Apr 12 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PGP9.5 antibody [EPR4118]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

投稿 Apr 12 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PGP9.5 antibody [EPR4118]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Jejunum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Jejunum
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

投稿 Dec 21 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PGP9.5 antibody [EPR4118]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Colon
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

投稿 Dec 21 2016

1-10 of 12 Abreviews or Q&A

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