Anti-PGM3 抗体 [OTI1A11] (ab128094)
Key features and details
- Mouse monoclonal [OTI1A11] to PGM3
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG1
製品の概要
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製品名
Anti-PGM3 antibody [OTI1A11]
PGM3 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [OTI1A11] to PGM3 -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-P, Flow Cyt (Intra)more details -
種交差性
交差種: Mouse, Human, African green monkey -
免疫原
Recombinant full length protein corresponding to Human PGM3. Produced in HKE293T cells (NP_056414).
Database link: O95394 -
ポジティブ・コントロール
- WB: HEK-293T cells transfected with pCMV6-ENTRY PGM3 cDNA; HepG2, HeLa, SVT2, COS-7 and MCF7 cell lysates. IHC-P: Human Kidney, Human liver, Human pancreas, Adenocarcinoma of Human endometrium, Human prostate and Carcinoma of Human prostate tissues. Flow Cyt (Intra): PGM3 transfected HEK-293T cells
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特記事項
The clone number has been updated from 1A11 to OTI1A11, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
Purified from cell culture supernatant by affinity chromatography -
ポリ/モノ
モノクローナル -
クローン名
OTI1A11 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab128094の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/2000. Predicted molecular weight: 60 kDa.
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IHC-P |
1/150.
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Flow Cyt (Intra) |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
1/500 - 1/2000. Predicted molecular weight: 60 kDa. |
IHC-P
1/150. |
Flow Cyt (Intra)
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Interconverts GlcNAc-6-P and GlcNAc-1-P. -
組織特異性
Found in many tissues except lung. Relatively high expression in pancreas, heart, liver, and placenta, and relatively low expression in brain, skeletal muscle and kidney. -
パスウェイ
Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I): step 2/2. -
配列類似性
Belongs to the phosphohexose mutase family. - Information by UniProt
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参照データベース
- Entrez Gene: 5238 Human
- Entrez Gene: 109785 Mouse
- Omim: 172100 Human
- SwissProt: O95394 Human
- SwissProt: Q9CYR6 Mouse
- Unigene: 661665 Human
- Unigene: 390201 Mouse
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別名
- 2810473H05Rik antibody
- Acetylglucosamine phosphomutase antibody
- Agm1 antibody
see all
画像
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All lanes : Anti-PGM3 antibody [OTI1A11] (ab128094) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY PGM3 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 60 kDa -
All lanes : Anti-PGM3 antibody [OTI1A11] (ab128094) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : SVT2 cell extract
Lane 4 : A549 (human lung carcinoma cell line) cell lysate
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 7 : MDCK cell extract
Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate
Lane 9 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 60 kDa -
Paraffin-embedded human kidney tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human liver tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human pancreas tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human endometrium adenocarcinoma tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human prostate tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human prosate carcinoma tissue stained for PGM3 using ab128094 at 1/150 dilution in immunohistochemical analysis.
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Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) using ab128094 at 1/100 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab128094 は 3 報の論文で使用されています。
- Ricciardiello F et al. Suppression of the HBP Function Increases Pancreatic Cancer Cell Sensitivity to a Pan-RAS Inhibitor. Cells 10:N/A (2021). PubMed: 33670598
- Li J et al. Correlative study on the JAK-STAT/PSMß3 signal transduction pathway in asthenozoospermia. Exp Ther Med 13:127-130 (2017). PubMed: 28123480
- Chaveroux C et al. Nutrient shortage triggers the hexosamine biosynthetic pathway via the GCN2-ATF4 signalling pathway. Sci Rep 6:27278 (2016). WB . PubMed: 27255611