Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6565] to PGD - BSA and Azide free
- Suitable for: Flow Cyt, ICC/IF, IP, WB
- Reacts with: Mouse, Rat, Human
製品名Anti-PGD antibody [EPR6565] - BSA and Azide free
PGD 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR6565] to PGD - BSA and Azide free
アプリケーション適用あり: Flow Cyt, ICC/IF, IP, WBmore details
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human PGD aa 400-500. The exact sequence is proprietary.
Ab248347 is the carrier-free version of ab129199. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248347 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
解離定数（KD 値）KD = 4.53 x 10 -11 M Learn more about KD
Concentration information loading...
Our Abpromise guarantee covers the use of ab248347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 53 kDa).|
機能Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.
パスウェイCarbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 3/3.
配列類似性Belongs to the 6-phosphogluconate dehydrogenase family.
- Information by UniProt
- 0610042A05Rik antibody
- 6 phosphogluconate dehydrogenase, decarboxylating antibody
- 6-phosphogluconate dehydrogenase antibody
All lanes : Anti-PGD antibody [EPR6565] (ab129199) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Molt-4 cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 53 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
This data was developed using ab129199, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.