Anti-PGAM1 抗体 (ab2220)

Reviews (1)Q&A (1)References (6)

製品の概要

  • 製品名

    Anti-PGAM1 antibody
    PGAM1 一次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal to PGAM1

  • 由来種

    Goat

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog, Pig, Monkey

  • 免疫原

    Synthetic peptide: KAMEAVAAQGKAKK, corresponding to C terminal amino acids 241-254 of Human PGAM1.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • Human liver and brain lysates.

  • 特記事項

    GenBank Accession Number – NP_002620.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 28 kDa.
IHC-P Use at an assay dependent concentration. PubMed: 21622897

ターゲット情報

画像

  • Western blot - Anti-PGAM1 antibody (ab2220)
    Western blot - Anti-PGAM1 antibody (ab2220)
    Anti-PGAM1 antibody (ab2220) at 0.01 µg/ml + Human liver lysate at 35 µg

    Developed using the ECL technique.


    Primary incubated for 1 hour

  • Western blot - Anti-PGAM1 antibody (ab2220)
    Western blot - Anti-PGAM1 antibody (ab2220)
    ab2220 staining (0.5µg/ml) of Human Liver lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. ab2220 staining (0.5µg/ml) of Human Liver lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM1 antibody (ab2220)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM1 antibody (ab2220)Image from Lei Y et al, Mol Cell Proteomics. 2011 Oct;10(10):M110.005397. Epub 2011 May 27, Fig 4. DOI 10.1074/mcp.M110.005397
    ab2220 staining PGAM1 in human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    N, normal colonic mucosa; TII, Stage II; and TIV, Stage IV. Positive staining is indicated by red arrows.

参考文献

This product has been referenced in:

  • Okuda J  et al. Persistent overexpression of phosphoglycerate mutase, a glycolytic enzyme, modifies energy metabolism and reduces stress resistance of heart in mice. PLoS One 8:e72173 (2013). WB ; Mouse . Read more (PubMed: 23951293) »
  • Hallows WC  et al. Regulation of Glycolytic Enzyme Phosphoglycerate Mutase-1 by Sirt1 Protein-mediated Deacetylation. J Biol Chem 287:3850-8 (2012). WB ; Human . Read more (PubMed: 22157007) »
See all 6 Publications for this product

ab2220 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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Western blot abreview for Anti-PGAM1 antibody

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Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (BRAIN)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
20 µg
Specification
BRAIN
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Nov 16 2015

Question

I wonder about specificity of that antibody. Does it really recognize only PGAM1 and not PGAM2 form?

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Answer

Thank you for your enquiry. I have blasted the immunizing peptide sequence and found that 13 out of　14 amino acids were identical to the sequence of PGAM2. Therefore, it is likely that this product might cross react with PGAM2. However, since we have not tested it in our laboratory, we are not sure of the results. I hope this information will be helpful. Should you require anything else, please do not hesitate to contact me.

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