Anti-PGAM1 抗体 (ab2220)
Key features and details
- Goat polyclonal to PGAM1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PGAM1 antibody
PGAM1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to PGAM1 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog, Pig, Monkey
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免疫原
Synthetic peptide corresponding to Human PGAM1 aa 200 to the C-terminus (C terminal). (NP_000281.2; NP_001025062.1)
Database link: NP_002620.1 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2220の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 28 kDa.
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
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WB
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 28 kDa. 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. -
組織特異性
In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues. -
配列類似性
Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily. - Information by UniProt
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参照データベース
- Entrez Gene: 404148 Cow
- Entrez Gene: 5223 Human
- Entrez Gene: 18648 Mouse
- Entrez Gene: 24642 Rat
- Omim: 172250 Human
- SwissProt: Q3SZ62 Cow
- SwissProt: P18669 Human
- SwissProt: Q9DBJ1 Mouse
see all -
別名
- BPG dependent PGAM 1 antibody
- BPG dependent PGAM1 antibody
- BPG-dependent PGAM 1 antibody
see all
画像
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Western blot - Anti-PGAM1 antibody (ab2220)Anti-PGAM1 antibody (ab2220) at 0.01 µg/ml + Human liver lysate at 35 µg
Developed using the ECL technique.Primary incubated for 1 hour
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Western blot - Anti-PGAM1 antibody (ab2220)ab2220 staining (0.5µ g/ml) of Human Liver lysate (RIPA buffer, 35µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ab2220 staining (0.5µg/ml) of Human Liver lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
データシートおよび資料
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Datasheet download
参考文献 (7)
ab2220 は 7 報の論文で使用されています。
- Huang K et al. A Novel Allosteric Inhibitor of Phosphoglycerate Mutase 1 Suppresses Growth and Metastasis of Non-Small-Cell Lung Cancer. Cell Metab 30:1107-1119.e8 (2019). PubMed: 31607564
- Okuda J et al. Persistent overexpression of phosphoglycerate mutase, a glycolytic enzyme, modifies energy metabolism and reduces stress resistance of heart in mice. PLoS One 8:e72173 (2013). WB ; Mouse . PubMed: 23951293
- Hallows WC et al. Regulation of Glycolytic Enzyme Phosphoglycerate Mutase-1 by Sirt1 Protein-mediated Deacetylation. J Biol Chem 287:3850-8 (2012). WB ; Human . PubMed: 22157007
- Lei Y et al. Proteomics Identification of ITGB3 as a Key Regulator in Reactive Oxygen Species-induced Migration and Invasion of Colorectal Cancer Cells. Mol Cell Proteomics 10:M110.005397 (2011). WB, IHC-P ; Human . PubMed: 21622897
- Klein-Scory S et al. Immunoscreening of the extracellular proteome of colorectal cancer cells. BMC Cancer 10:70 (2010). WB ; Human . PubMed: 20184735
- Evans MJ et al. Mechanistic and structural requirements for active site labeling of phosphoglycerate mutase by spiroepoxides. Mol Biosyst 3:495-506 (2007). PubMed: 17579775
- Evans MJ et al. Target discovery in small-molecule cell-based screens by in situ proteome reactivity profiling. Nat Biotechnol 23:1303-7 (2005). PubMed: 16200062
