Anti-PGAM1 抗体 (ab2220)
製品の概要
-
製品名
Anti-PGAM1 antibody
PGAM1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to PGAM1 -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Dog, Pig, Monkey -
免疫原
Synthetic peptide: KAMEAVAAQGKAKK, corresponding to C terminal amino acids 241-254 of Human PGAM1.
-
ポジティブ・コントロール
- Human liver and brain lysates.
-
特記事項
GenBank Accession Number – NP_002620.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab2220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 28 kDa. | |
IHC-P | Use at an assay dependent concentration. PubMed: 21622897 |
ターゲット情報
-
機能
Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. -
組織特異性
In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues. -
配列類似性
Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily. - Information by UniProt
-
参照データベース
- Entrez Gene: 404148 Cow
- Entrez Gene: 5223 Human
- Entrez Gene: 18648 Mouse
- Entrez Gene: 24642 Rat
- Omim: 172250 Human
- SwissProt: Q3SZ62 Cow
- SwissProt: P18669 Human
- SwissProt: Q9DBJ1 Mouse
see all -
別名
- BPG dependent PGAM 1 antibody
- BPG dependent PGAM1 antibody
- BPG-dependent PGAM 1 antibody
see all
画像
-
Anti-PGAM1 antibody (ab2220) at 0.01 µg/ml + Human liver lysate at 35 µg
Developed using the ECL technique.Primary incubated for 1 hour
-
ab2220 staining (0.5
µ g/ml) of Human Liver lysate (RIPA buffer, 35µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ab2220 staining (0.5µg/ml) of Human Liver lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGAM1 antibody (ab2220)Image from Lei Y et al, Mol Cell Proteomics. 2011 Oct;10(10):M110.005397. Epub 2011 May 27, Fig 4. DOI 10.1074/mcp.M110.005397ab2220 staining PGAM1 in human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
N, normal colonic mucosa; TII, Stage II; and TIV, Stage IV. Positive staining is indicated by red arrows.
データシートおよび資料
参考文献
This product has been referenced in:
- Okuda J et al. Persistent overexpression of phosphoglycerate mutase, a glycolytic enzyme, modifies energy metabolism and reduces stress resistance of heart in mice. PLoS One 8:e72173 (2013). WB ; Mouse . Read more (PubMed: 23951293) »
- Hallows WC et al. Regulation of Glycolytic Enzyme Phosphoglycerate Mutase-1 by Sirt1 Protein-mediated Deacetylation. J Biol Chem 287:3850-8 (2012). WB ; Human . Read more (PubMed: 22157007) »