Anti-PGAM1 抗体 (ab2220)

Reviews (1)Q&A (1)References (6)

Key features and details

  • Goat polyclonal to PGAM1
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-PGAM1 antibody
    PGAM1 一次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal to PGAM1

  • 由来種

    Goat

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog, Pig, Monkey

  • 免疫原

    Synthetic peptide: KAMEAVAAQGKAKK, corresponding to C terminal amino acids 241-254 of Human PGAM1.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • Human liver and brain lysates.

  • 特記事項

    GenBank Accession Number – NP_002620.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 28 kDa.

1 hour primary incubation is recommended for this product.

ターゲット情報

画像

  • Western blot - Anti-PGAM1 antibody (ab2220)
    Western blot - Anti-PGAM1 antibody (ab2220)
    Anti-PGAM1 antibody (ab2220) at 0.01 µg/ml + Human liver lysate at 35 µg

    Developed using the ECL technique.


    Primary incubated for 1 hour

  • Western blot - Anti-PGAM1 antibody (ab2220)
    Western blot - Anti-PGAM1 antibody (ab2220)
    ab2220 staining (0.5µg/ml) of Human Liver lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. ab2220 staining (0.5µg/ml) of Human Liver lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

参考文献

ab2220 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab2220 は 6 報の論文で使用されています。

  • Okuda J  et al. Persistent overexpression of phosphoglycerate mutase, a glycolytic enzyme, modifies energy metabolism and reduces stress resistance of heart in mice. PLoS One 8:e72173 (2013). WB ; Mouse . PubMed: 23951293
  • Hallows WC  et al. Regulation of Glycolytic Enzyme Phosphoglycerate Mutase-1 by Sirt1 Protein-mediated Deacetylation. J Biol Chem 287:3850-8 (2012). WB ; Human . PubMed: 22157007
  • Lei Y  et al. Proteomics Identification of ITGB3 as a Key Regulator in Reactive Oxygen Species-induced Migration and Invasion of Colorectal Cancer Cells. Mol Cell Proteomics 10:M110.005397 (2011). WB, IHC-P ; Human . PubMed: 21622897
  • Klein-Scory S  et al. Immunoscreening of the extracellular proteome of colorectal cancer cells. BMC Cancer 10:70 (2010). WB ; Human . PubMed: 20184735
  • Evans MJ  et al. Mechanistic and structural requirements for active site labeling of phosphoglycerate mutase by spiroepoxides. Mol Biosyst 3:495-506 (2007). PubMed: 17579775
  • Evans MJ  et al. Target discovery in small-molecule cell-based screens by in situ proteome reactivity profiling. Nat Biotechnol 23:1303-7 (2005). PubMed: 16200062

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Western blot abreview for Anti-PGAM1 antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (BRAIN)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
20 µg
Specification
BRAIN
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Nov 16 2015

Question

I wonder about specificity of that antibody. Does it really recognize only PGAM1 and not PGAM2 form?

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Answer

Thank you for your enquiry. I have blasted the immunizing peptide sequence and found that 13 out of　14 amino acids were identical to the sequence of PGAM2. Therefore, it is likely that this product might cross react with PGAM2. However, since we have not tested it in our laboratory, we are not sure of the results. I hope this information will be helpful. Should you require anything else, please do not hesitate to contact me.

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