Anti-PF4 抗体 [EPR7763] (ab129183)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7763] to PF4
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PF4 antibody [EPR7763]
PF4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7763] to PF4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt (Intra) or ICC/IF -
種交差性
交差種: Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Human spleen and platelet lysates, Human spleen tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
解離定数(KD 値)
KD = 5.40 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7763 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab129183の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 11 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻訳後修飾
Binds non-covalently to a proteoglycan molecule. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 5196 Human
- Entrez Gene: 360918 Rat
- Omim: 173460 Human
- SwissProt: P02776 Human
- SwissProt: P06765 Rat
- Unigene: 81564 Human
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別名
- C-X-C motif chemokine 4 antibody
- Chemokine (C X C motif) ligand 4 antibody
- Chemokine (CXC motif) ligand 4 antibody
see all
画像
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ab129183, at a 1/100 dilution, staining PF4 in paraffin embedded Human spleen tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of parafifin-embedded sections of human spleen tissue with primary antibody ab129183 at 1/200 dilution and incubated at +4°C overnight, followed by secondary antibody Goat Anti-Rabbit IgG H&L (HRP polymer). Platelet staining on human spleen is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval using Antigen Retrieval Buffer (citrate buffer, pH 6.0) ab93678.
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Immunohistochemical analysis of parafifin-embedded sections of mouse liver tissue with primary antibody ab129183 at 1/200 dilution and incubated at +4°C overnight, followed by secondary antibody Goat Anti-Rabbit IgG H&L (HRP polymer). Sporadically platelet staining on mouse liver is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval using Antigen Retrieval Buffer (citrate buffer, pH 6.0) ab93678.
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Immunohistochemical analysis of parafifin-embedded sections of rat liver tissue with primary antibody ab129183 at 1/200 dilution and incubated at +4°C overnight, followed by secondary antibody Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on rat liver is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval using Antigen Retrieval Buffer (citrate buffer, pH 6.0) ab93678.
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All lanes : Anti-PF4 antibody [EPR7763] (ab129183) at 1/1000 dilution
Lane 1 : Human spleen lysates
Lane 2 : Human platelet lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 11 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab129183 は 4 報の論文で使用されています。
- Kriek N et al. Cucurbitacins Elicit Anti-Platelet Activity via Perturbation of the Cytoskeleton and Integrin Function. Thromb Haemost 122:1115-1129 (2022). PubMed: 35253142
- Zhang X et al. Comparison of serum and plasma as a source of blood extracellular vesicles: Increased levels of platelet-derived particles in serum extracellular vesicle fractions alter content profiles from plasma extracellular vesicle fractions. PLoS One 17:e0270634 (2022). PubMed: 35749554
- Chacón-Fernández P et al. Brain-derived Neurotrophic Factor in Megakaryocytes. J Biol Chem 291:9872-81 (2016). PubMed: 27006395
- Li J et al. CXCL4 Contributes to the Pathogenesis of Chronic Liver Allograft Dysfunction. J Immunol Res 2016:9276986 (2016). IHC . PubMed: 28053995