Anti-PF4 抗体 (ab9561)
Key features and details
- Rabbit polyclonal to PF4
- Suitable for: WB, Sandwich ELISA
- Reacts with: Human
- Isotype: unknown
製品の概要
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製品名
Anti-PF4 antibody
PF4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PF4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, Sandwich ELISAmore details -
種交差性
交差種: Human -
免疫原
Highly pure (>98%) recombinant hPF-4 (human Platelet Factor-4)
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ポジティブ･コントロール
- Recombinant human PF4 protein (ab9562) can be used as a positive control in WB.
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特記事項
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab49735
製品の特性
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製品の状態
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
unknown -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab9561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. | |
| Sandwich ELISA | Use at an assay dependent concentration. Can be paired for Sandwich ELISA with ab83123 |
ターゲット情報
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機能
Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻訳後修飾
Binds non-covalently to a proteoglycan molecule. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 5196 Human
- Omim: 173460 Human
- SwissProt: P02776 Human
- Unigene: 81564 Human
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別名
- C-X-C motif chemokine 4 antibody
- Chemokine (C X C motif) ligand 4 antibody
- Chemokine (CXC motif) ligand 4 antibody
see all
画像
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Western blot - Anti-PF4 antibody (ab9561)Anti-PF4 antibody (ab9561) at 1 µg/ml + Human Platelet (Human adult normal cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 11 kDa
Observed band size: 11 kDa
Additional bands at: 18 kDa, 30 kDa, 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes -
Sandwich ELISA - Anti-PF4 antibody (ab9561)To detect Human PF-4 by sandwich ELISA (using 100μl/well) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with suitable detection antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human PF-4.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (9)
ab9561 は 9 報の論文で使用されています。
- Chanpeng P et al. Platelet proteome reveals specific proteins associated with platelet activation and the hypercoagulable state in ß-thalassmia/HbE patients. Sci Rep 9:6059 (2019). PubMed: 30988349
- Lande R et al. CXCL4 assembles DNA into liquid crystalline complexes to amplify TLR9-mediated interferon-a production in systemic sclerosis. Nat Commun 10:1731 (2019). PubMed: 31043596
- Gollomp K et al. Neutrophil accumulation and NET release contribute to thrombosis in HIT. JCI Insight 3:N/A (2018). PubMed: 30232279
- Maybin JA et al. Steroids Regulate CXCL4 in the Human Endometrium During Menstruation to Enable Efficient Endometrial Repair. J Clin Endocrinol Metab 102:1851-1860 (2017). PubMed: 28323919
- Izumi M et al. Reduced induction of anti-PF4/heparin antibody in RA patients after total knee arthroplasty. Arthritis Res Ther 18:191 (2016). Human . PubMed: 27558507
- Du Y et al. Wnt3a is critical for endothelial progenitor cell-mediated neural stem cell proliferation and differentiation. Mol Med Rep 14:2473-82 (2016). WB . PubMed: 27484039
- Fritzer A et al. Chemokine degradation by the Group A streptococcal serine proteinase ScpC can be reconstituted in vitro and requires two separate domains. Biochem J 422:533-42 (2009). WB . PubMed: 19552626
- Houard X et al. Mediators of neutrophil recruitment in human abdominal aortic aneurysms. Cardiovasc Res 82:532-41 (2009). IHC-P ; Human . PubMed: 19201759
- Lambert MP et al. Platelet factor 4 is a negative autocrine in vivo regulator of megakaryopoiesis: clinical and therapeutic implications. Blood 110:1153-60 (2007). Neutralising ; Human . PubMed: 17495129
