Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7885] to PERP - BSA and Azide free
- Suitable for: ICC/IF, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
製品名Anti-PERP antibody [EPR7885] - BSA and Azide free
PERP 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR7885] to PERP - BSA and Azide free
アプリケーション適用あり: ICC/IF, WBmore details
適用なし: Flow Cyt,IHC-P or IP
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human PERP aa 150-250 (C terminal). The exact sequence is proprietary.
Database link: Q96FX8
- WB: HeLa and HaCaT cell lysates.
Ab248288 is the carrier-free version of ab129083. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248288 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
解離定数（KD 値）KD = 4.10 x 10 -11 M Learn more about KD
Concentration information loading...
KO cell lines
KO cell lysates
Our Abpromise guarantee covers the use of ab248288 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 21 kDa.|
機能Component of intercellular desmosome junctions. Plays a role in stratified epithelial integrity and cell-cell adhesion by promoting desmosome assembly. Plays a role as an effector in the TP53-dependent apoptotic pathway.
組織特異性Expressed in skin, heart, placental, liver, pancreas, keratinocytes and dermal fibroblasts.
配列類似性Belongs to the TMEM47 family.
細胞内局在Cell junction > desmosome. Cell membrane. Associated with desmosomes.
- Information by UniProt
- 1110017A08Rik antibody
- dJ496H19.1 antibody
- KCP 1 antibody
All lanes : Anti-PERP antibody [EPR7885] (ab129083) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PERP knockout HeLa cell lysate
Lane 3 : HaCaT cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
This data was developed using the same antibody clone in a different buffer formulation (ab129083).
ab129083 Anti-PERP antibody [EPR7885] was shown to specifically react with PERP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265335 (knockout cell lysate ab258105) was used. Wild-type and PERP knockout samples were subjected to SDS-PAGE. ab129083 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.