Anti-PERP 抗体 (ab5986)
Key features and details
- Rabbit polyclonal to PERP
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PERP antibody
PERP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PERP -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide:
EDDLLGNAKPRYFYTSA
, corresponding to amino acids 175-193 of Human PERP. -
特記事項
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in the p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2, MTBP and PERP, were identified and their genes were cloned recently. PERP, also termed PIGPC1 and THW, is a plasma membrane protein. p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis. The expression of PERP causes cell death. PERP is a mediator of p53 induced apoptosis. PERP has sequence similarity to PMP-22/gas3 and is a new member of the PMP-22/gas3 family.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
一次抗体 備考
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in the p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2, MTBP and PERP, were identified and their genes were cloned recently. PERP, also termed PIGPC1 and THW, is a plasma membrane protein. p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis. The expression of PERP causes cell death. PERP is a mediator of p53 induced apoptosis. PERP has sequence similarity to PMP-22/gas3 and is a new member of the PMP-22/gas3 family. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab5986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | Use a concentration of 10 µg/ml. | |
WB | Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 21 kDa. |
ターゲット情報
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機能
Component of intercellular desmosome junctions. Plays a role in stratified epithelial integrity and cell-cell adhesion by promoting desmosome assembly. Plays a role as an effector in the TP53-dependent apoptotic pathway. -
組織特異性
Expressed in skin, heart, placental, liver, pancreas, keratinocytes and dermal fibroblasts. -
配列類似性
Belongs to the TMEM47 family. -
細胞内局在
Cell junction > desmosome. Cell membrane. Associated with desmosomes. - Information by UniProt
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参照データベース
- Entrez Gene: 64065 Human
- Entrez Gene: 64058 Mouse
- Omim: 609301 Human
- SwissProt: Q96FX8 Human
- SwissProt: Q9JK95 Mouse
- Unigene: 201446 Human
- Unigene: 28209 Mouse
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別名
- 1110017A08Rik antibody
- dJ496H19.1 antibody
- KCP 1 antibody
see all
画像
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All lanes : Anti-PERP antibody (ab5986) at 1 µg
Lane 1 : 293 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : K569 cell lysate
Lane 6 : MCF-7 cell lysate
Lane 7 : SK-N-SH cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution -
Western blot analysis of PERP expression in A431 whole cell lysates in the absence (A) and presence (B) of blocking peptide with anti- PERP at 1
µ g /ml. Western blot analysis of PERP expression in A431 whole cell lysates in the absence (A) and presence (B) of blocking peptide with anti- PERP at 1 µg /ml. -
Immunocytochemistry of PERP in A431 cells with PERP antibody at 10 µg/ml.
プロトコール
データシートおよび資料
参考文献 (7)
ab5986 は 7 報の論文で使用されています。
- McDonnell SJ et al. ER stress-linked autophagy stabilizes apoptosis effector PERP and triggers its co-localization with SERCA2b at ER-plasma membrane junctions. Cell Death Discov 5:132 (2019). PubMed: 31508245
- Awais R et al. p63 is required beside p53 for PERP-mediated apoptosis in uveal melanoma. Br J Cancer 115:983-992 (2016). WB . PubMed: 27584665
- Marthandan S et al. Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts. Biomed Res Int 2015:731938 (2015). WB . PubMed: 26339636
- Hatanpaa KJ et al. High expression of the stem cell marker nestin is an adverse prognostic factor in WHO grade II-III astrocytomas and oligoastrocytomas. J Neurooncol 117:183-189 (2014). PubMed: 24519516
- Teh MT et al. A molecular study of desmosomes identifies a desmoglein isoform switch in head and neck squamous cell carcinoma. J Oral Pathol Med 40:67-76 (2011). WB ; Human . PubMed: 20923451
- Ding Z et al. Preferential gene expression in the limbus of the vervet monkey. Mol Vis 14:2031-41 (2008). IHC-Fr ; Monkey . PubMed: 18989386
- Paraoan L et al. Expression of p53-induced apoptosis effector PERP in primary uveal melanomas: downregulation is associated with aggressive type. Exp Eye Res 83:911-9 (2006). PubMed: 16784742