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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Peroxiredoxin 5 抗体 [EPR14529(B)] (ab180587)

Submit a review Submit a question References (4)
Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
  • Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
  • Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
  • Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14529(B)] to Peroxiredoxin 5
  • Suitable for: WB, IHC-P, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Peroxiredoxin 5 antibody [EPR14529(B)]
    Peroxiredoxin 5 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14529(B)] to Peroxiredoxin 5

  • 由来種

    Rabbit

  • 特異性

    The mouse and  rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • アプリケーション

    適用あり: WB, IHC-P, IPmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HeLa, HepG2 and A549 cell line lysates; Human kidney tissue; Jurkat cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR14529(B)

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab180587の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 22 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The mouse and  rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

See IHC antigen retrieval protocols
IP
1/50.
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 22 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The mouse and  rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

See IHC antigen retrieval protocols
IP
1/50.

ターゲット情報

  • 機能

    Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling.

  • 組織特異性

    Widely expressed.

  • 配列類似性

    Belongs to the peroxiredoxin 2 family.
    Contains 1 thioredoxin domain.

  • 細胞内局在

    Mitochondrion. Cytoplasm. Peroxisome.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • ACR1 antibody
    • Alu co repressor 1 antibody
    • Alu corepressor 1 antibody
    • Antioxidant enzyme B166 antibody
    • AOEB166 antibody
    • B166 antibody
    • epididymis secretory protein Li 55 antibody
    • HEL-S-55 antibody
    • Liver tissue 2D-page spot 71B antibody
    • mitochondrial antibody
    • Peroxiredoxin 5, mitochondrial antibody
    • Peroxiredoxin V antibody
    • Peroxiredoxin-5 antibody
    • Peroxisomal antioxidant enzyme antibody
    • PLP antibody
    • PMP20 antibody
    • PRDX 5 antibody
    • PRDX5 antibody
    • PRDX5_HUMAN antibody
    • PRDX6 antibody
    • Prx-V antibody
    • PRXV antibody
    • SBBI10 antibody
    • Thioredoxin peroxidase PMP20 antibody
    • Thioredoxin reductase antibody
    • TPx type VI antibody
    see all

画像

  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: PRDX5 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HepG2 whole cell lysate (20 µg)
    Lane 4: A549 whole cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab180587 observed at 17 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab180587 was shown to specifically react with PRDX5 when PRDX5 knockout samples were used. Wild-type and PRDX5 knockout samples were subjected to SDS-PAGE. Ab180587 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling Peroxiredoxin 5 with purified ab180587 at 1:500 dilution (1.18 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    ab180587 (Purified) at 1:30 dilution (2 µg) immunoprecipitating Peroxiredoxin 5 in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
    Lane 2 (+): ab180587 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab180587 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.
  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    All lanes : Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/10000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Human brain lysates
    Lane 3 : Rat brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 22 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/5000 dilution (Purified) + Mouse brain lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 22 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)

    Immunoprecipitation analysis of Peroxiredoxin 5 pulled down from HeLa cell lysates using ab180587 at 1/50 dilution.



    Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/1000 dilution (Unpurified) + HeLa cell immunoprecipitated with ab180587 at 1/50.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Observed band size: 17 kDa why is the actual band size different from the predicted?
    Additional bands at: 50 kDa (possible IgG)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)

    Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling Peroxiredoxin 5 with unpurified ab180587 at 1/250.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    All lanes : Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/5000 dilution (Unpurified)

    Lane 1 : HepG2 cell line lysate
    Lane 2 : Jurkat cell line lysate
    Lane 3 : A549 cell line lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/20000 dilution (Unpurified) + HeLa cell line lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?

  • Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)

参考文献 (4)

ab180587 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab180587 は 4 報の論文で使用されています。

  • Cao X  et al. ROS-mediated hypomethylation of PRDX5 promotes STAT3 binding and activates the Nrf2 signaling pathway in NSCLC. Int J Mol Med 47:573-582 (2021). PubMed: 33416106
  • Gao J  et al. Metformin protects against PM2.5-induced lung injury and cardiac dysfunction independent of AMP-activated protein kinase a2. Redox Biol 28:101345 (2020). PubMed: 31669973
  • Brown LA  et al. Late life maintenance and enhancement of functional exercise capacity in low and high responding rats after low intensity treadmill training. Exp Gerontol 125:110657 (2019). PubMed: 31306740
  • Wang H  et al. AMPKa2 deficiency exacerbates long-term PM2.5 exposure-induced lung injury and cardiac dysfunction. Free Radic Biol Med 121:202-214 (2018). PubMed: 29753072

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