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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Peroxiredoxin 5 抗体 [EPR14528(B)] (ab180123)

Submit a review Submit a question References (1)
Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)
  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)
  • Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)
  • Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)
  • Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)
  • Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14528(B)] to Peroxiredoxin 5
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Peroxiredoxin 5 antibody [EPR14528(B)]
    Peroxiredoxin 5 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14528(B)] to Peroxiredoxin 5

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, ICC/IF, IPmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human Peroxiredoxin 5 aa 50-150. The exact sequence is proprietary.
    Database link: P30044

  • ポジティブ･コントロール

    • HepG2, HeLa, Jurkat and A549 cell lysates; HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR14528(B)

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab180123の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/20000. Detects a band of approximately 17 kDa (predicted molecular weight: 22 kDa).
ICC/IF
1/250.
IP
1/60.
特記事項
Flow Cyt (Intra)
1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/20000. Detects a band of approximately 17 kDa (predicted molecular weight: 22 kDa).
ICC/IF
1/250.
IP
1/60.

ターゲット情報

  • 機能

    Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling.

  • 組織特異性

    Widely expressed.

  • 配列類似性

    Belongs to the peroxiredoxin 2 family.
    Contains 1 thioredoxin domain.

  • 細胞内局在

    Mitochondrion. Cytoplasm. Peroxisome.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • ACR1 antibody
    • Alu co repressor 1 antibody
    • Alu corepressor 1 antibody
    • Antioxidant enzyme B166 antibody
    • AOEB166 antibody
    • B166 antibody
    • epididymis secretory protein Li 55 antibody
    • HEL-S-55 antibody
    • Liver tissue 2D-page spot 71B antibody
    • mitochondrial antibody
    • Peroxiredoxin 5, mitochondrial antibody
    • Peroxiredoxin V antibody
    • Peroxiredoxin-5 antibody
    • Peroxisomal antioxidant enzyme antibody
    • PLP antibody
    • PMP20 antibody
    • PRDX 5 antibody
    • PRDX5 antibody
    • PRDX5_HUMAN antibody
    • PRDX6 antibody
    • Prx-V antibody
    • PRXV antibody
    • SBBI10 antibody
    • Thioredoxin peroxidase PMP20 antibody
    • Thioredoxin reductase antibody
    • TPx type VI antibody
    see all

画像

  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)
    Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: PRDX5 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HepG2 whole cell lysate (20 µg)
    Lane 4: A549 whole cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab180123 observed at 17 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab180123 was shown to specifically react with PRDX5 when PRDX5 knockout samples were used. Wild-type and PRDX5 knockout samples were subjected to SDS-PAGE. Ab180123 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 10000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)
    Western blot - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)
    All lanes : Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123) at 1/20000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa
    Additional bands at: 17 kDa. We are unsure as to the identity of these extra bands.

  • Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)
    Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Peroxiredoxin 5 with ab180123 at 1/250 dilution, followed by Goat anti rabbit IgG (Dylight 555) at 1/250 dilution. Counterstained with DAPI (blue).

  • Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)
    Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)

    Western Blot analysis of HeLa cell lysate immunoprecipitated with ab180123 at 1/60 dilution, using Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)
    Flow Cytometry (Intracellular) - Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)

    Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Peroxiredoxin 5with ab180123 at 1/10 dilution (red) compared toa rabbit IgG control (green), followed by Goat anti rabbit IgG (FITC) at 1/75 dilution. 

     

     

  • Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)
    Anti-Peroxiredoxin 5 antibody [EPR14528(B)] (ab180123)

参考文献 (1)

ab180123 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab180123 は 1 報の論文で使用されています。

  • Lovatt M  et al. Peroxiredoxin-1 regulates lipid peroxidation in corneal endothelial cells. Redox Biol 30:101417 (2020). PubMed: 31901729

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