製品の概要

  • 製品名
    Anti-Peroxiredoxin 1/PAG antibody
    Peroxiredoxin 1/PAG 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Peroxiredoxin 1/PAG
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, IHC-P, IP, WBmore details
  • 種交差性
    交差種: Mouse, Rabbit, Human
    交差が予測される動物種: Rat, Cow
  • 免疫原

    Synthetic peptide corresponding to Human Peroxiredoxin 1/PAG aa 100 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab41919, ab41920)

  • ポジティブ・コントロール
    • WB: Recombinant human Peroxiredoxin 1/PAG protein (ab74172), HeLa, A431, Jurkat, HEK293, HepG2, MCF7, SHSY-5Y and U20S whole cell lysates. ICC/IF: HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab41906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 20631257
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 22 kDa).

ターゲット情報

  • 機能
    Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.
  • 配列類似性
    Belongs to the ahpC/TSA family.
    Contains 1 thioredoxin domain.
  • 翻訳後修飾
    Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.
  • 細胞内局在
    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Heme binding 23 kDa protein antibody
    • MSP23 antibody
    • Natural killer cell-enhancing factor A antibody
    • NKEF A antibody
    • NKEF-A antibody
    • NKEFA antibody
    • OSF3 antibody
    • Osteoblast specific factor 3 antibody
    • PAG antibody
    • Paga antibody
    • PAGB antibody
    • Peroxiredoxin 1 antibody
    • Peroxiredoxin-1 antibody
    • PRDX1 antibody
    • PRDX1_HUMAN antibody
    • Proliferation associated gene A antibody
    • Proliferation-associated gene protein antibody
    • PRX1 antibody
    • PrxI antibody
    • TDPX2 antibody
    • Thioredoxin peroxidase 2 antibody
    • Thioredoxin-dependent peroxide reductase 2 antibody
    see all

画像

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Peroxiredoxin 1/PAG knockout HAP1 cell lysate (20 µg)
    Lane 3: A431 cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab41906 observed at 23 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab41906 was shown to recognize Peroxiredoxin 1/PAG when Peroxiredoxin 1/PAG knockout samples were used, along with additional cross-reactive bands. Wild-type and Peroxiredoxin 1/PAG knockout samples were subjected to SDS-PAGE. ab41906 and ab8245 (loading control to GAPDH) at a concentration of 1 µg/ml  and diluted to  1/10000 respectively were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-Peroxiredoxin 1/PAG antibody (ab41906) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 24 kDa
    why is the actual band size different from the predicted?

  • IHC image of Peroxiredoxin 1/PAG staining in human liver carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab41906, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • This protein is a homodimer consisting of two subunits with an expected molecular weight of 23kDa.
  • ICC/IF image of ab41906 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41906, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Morita K  et al. Peroxiredoxin as a functional endogenous antioxidant enzyme in pronuclei of mouse zygotes. J Reprod Dev 64:161-171 (2018). Read more (PubMed: 29503398) »
  • Jeong SJ  et al. Prdx1 (peroxiredoxin 1) deficiency reduces cholesterol efflux via impaired macrophage lipophagic flux. Autophagy 14:120-133 (2018). Read more (PubMed: 28605287) »
See all 20 Publications for this product

レビューと Q&A

1-6 of 6 Abreviews or Q&A

Application
Western blot
Sample
Rat Tissue lysate - whole (Aorta)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
50 µg
Treatment
Calcification Diet + Vitamin Ð3
Specification
Aorta
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Dr. D Richard

Verified customer

投稿 Jul 27 2015

Answer

Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que le produit ab15571 que vous avez reçu ne fonctionne pas comme attendu.
Le numéro de commande de remplacement gratuitavec leproduit ab41906 est *****. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

N'hésitez pas à nous contacter lors d'une prochaine occasion.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Hippocampus neuronal cells HpL3-4)
Gel Running Conditions
Non-reduced Denaturing (12%)
Loading amount
20 µg
Specification
Hippocampus neuronal cells HpL3-4
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C

Abcam user community

Verified customer

投稿 Sep 28 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TritonX100 in PBS

Dr. Kirk Mcmanus

Verified customer

投稿 Nov 16 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (MDA231)
Total protein in input
500 µg
Immuno-precipitation step
Protein G
Specification
MDA231

Xumei Wang

Verified customer

投稿 Aug 28 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (heart)
Loading amount
30 µg
Specification
heart
Gel Running Conditions
Non-reduced Denaturing (12% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C

Abcam user community

Verified customer

投稿 May 09 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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