Anti-Peroxiredoxin 1/PAG 抗体 (ab15571)
製品の概要
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製品名Anti-Peroxiredoxin 1/PAG antibody
Peroxiredoxin 1/PAG 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Peroxiredoxin 1/PAG
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由来種Rabbit
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特異性This antibody detects Peroxiredoxin 1 protein in human samples. The antibody is specific for Peroxiredoxin 1/PAG and shows no cross reactivity with other Prx isoforms.
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アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
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種交差性交差種: Mouse, Rat, Human, African green monkey
交差が予測される動物種: Cow, Chinese hamster
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免疫原
Synthetic peptide corresponding to Human Peroxiredoxin 1/PAG aa 103-114.
Sequence:LVSDPKRTIAQD
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ポジティブ･コントロール
- Recombinant human Peroxiredoxin 1/PAG protein (ab74172) can be used as a positive control in WB. TSU Pr1 cells for cell staining, human PC3 cell lysate for western blotting.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.05% Sodium azide
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精製度Whole antiserum
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab15571 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | 1/200. | |
| IHC-P | Use at an assay dependent concentration. | |
| WB | 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 22 kDa). |
ターゲット情報
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機能Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.
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配列類似性Belongs to the ahpC/TSA family.
Contains 1 thioredoxin domain. -
翻訳後修飾Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.
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細胞内局在Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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参照データベース
- Entrez Gene: 281997 Cow
- Entrez Gene: 5052 Human
- Entrez Gene: 18477 Mouse
- Entrez Gene: 117254 Rat
- Omim: 176763 Human
- SwissProt: Q9JKY1 Chinese hamster
- SwissProt: Q5E947 Cow
- SwissProt: Q06830 Human
see all -
別名
- Heme binding 23 kDa protein antibody
- MSP23 antibody
- Natural killer cell-enhancing factor A antibody
see all
画像
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Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab15571)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Peroxiredoxin 1/PAG knockout HAP1 cell lysate (20 µg)
Lane 3: A431 cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab15571 observed at 23 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab15571 was shown to recognize Peroxiredoxin 1/PAG when Peroxiredoxin 1/PAG knockout samples were used, along with additional cross-reactive bands. Wild-type and Peroxiredoxin 1/PAG knockout samples were subjected to SDS-PAGE. ab15571 and ab8245 (loading control to GAPDH) were diluted to 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) andGoat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 1/PAG antibody (ab15571)ICC/IF image of ab15571 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15571, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 1/PAG antibody (ab15571)IHC image of ab15571 staining in human liver carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab15571, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-Peroxiredoxin 1/PAG antibody (ab15571)All lanes : Anti-Peroxiredoxin 1/PAG antibody (ab15571) at 1/1000 dilution
Lane 1 : Cell line indicated at 25 µg
Secondary
All lanes : HRP-conjugated Goat anti-rabbit at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa. We are unsure as to the identity of these extra bands.Western blot analysis of Peroxiredoxin 1//PAG was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with ab15571 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody for at least one hour. Membranes were washed and chemiluminescent detection performed.
データシートおよび資料
プロトコール
参考文献
This product has been referenced in:
- Li HX et al. Peroxiredoxin 1 promoted tumor metastasis and angiogenesis in colorectal cancer. Pathol Res Pract 214:655-660 (2018). Read more (PubMed: 29673884) »
- Iqbal J et al. Selenium positively affects the proteome of 3 × Tg-AD mice cortex by altering the expression of various key proteins: unveiling the mechanistic role of selenium in AD prevention. J Neurosci Res 96:1798-1815 (2018). Read more (PubMed: 30054946) »
