Peroxide Assay Kit (ab272537)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Cell culture media, Cell Lysate, Other biological fluids, Plasma, Serum, Urine
製品の概要
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製品名
Peroxide Assay Kit
Hydrogen Peroxide キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media -
アッセイタイプ
Quantitative -
検出範囲
0.2 µM - 30 µM -
製品の概要
Peroxide Assay Kit (ab272537) is a simple, direct and automation-ready procedures for quantitative determination of peroxide find wide applications in research and drug discovery. This assay kit is designed to measure peroxide concentration in biological samples without any pretreatment. The improved method utilizes the chromogenic Fe3+-xylenol orange reaction, in which a purple complex is formed when Fe2+ provided in the reagent is oxidized to Fe3+ by peroxides present in the sample. The intensity of the color, measured at 540-610nm, is an accurate measure of the peroxide level in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.
Sensitive and accurate. Enhanced color intensity using sorbitol. Detection range 0.2 µM (7 ng/mL) to 30 µM (1,020 ng/mL) H2O2 in 96-well plate assay.
Simple and high-throughput. The procedure involves addition of a single detection reagent and incubation for 30 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.
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アプリケーション
適用あり: Functional Studiesmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 250 tests Reagent A 1 x 1ml Reagent B 1 x 50ml Standard (3% stabilized H2O2) 1 x 100µl -
研究分野
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関連性
Hydrogen Peroxide (H2O2) is a reactive oxygen metabolic byproduct that serves as a key regulator for a number of oxidative stress-related states. Functioning through NF-kB and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neuro-degenerative diseases, Down’s syndrome and immune system diseases. -
別名
- H2O2
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab272537の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Functional Studies |
Use at an assay dependent concentration.
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特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab272537 は論文での使用が確認できていません。