製品の概要

  • 製品名
    Peroxidase Activity Assay Kit
  • 検出方法
    Colorimetric/Fluorometric
  • サンプルの種類
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids
  • アッセイタイプ
    Enzyme activity
  • 種交差性
    交差種: Mammals, Other species
  • 製品の概要

    Abcam’s Peroxidase Assay Kit (ab155895) provides a convenient colorimetric and fluorometric means to measure the peroxidase activity in biological samples. In the presence of Peroxidase, the OxiRed Probe reacts with H2O2 in a 1:1 stoichiometry to produce the redfluorescent oxidation product, resorufin. The resorufin is quantified by colorimetric (λmax = 570nm) or fluorometric methods (Ex/Em = 535/587 nm). The assay is simple, direct, highly sensitive and high throughput-ready. The detection limit is 0.1 mU per well via colorimetric or 0.01 mU per well via fluorometric method, based on our unit definition.

  • 特記事項

    Peroxidases (EC number 1.11.1.x) are a large family of enzymes that typically catalyze a reaction of the form: ROOR' + electron donor (2 e-) + 2H+ → ROH + R'OH. For many of these enzymes the optimal substrate is hydrogen peroxide, but others are more active with organic hydroperoxides such as lipid peroxides. Peroxidases can contain a heme cofactor in their active sites, or alternately redox-active cysteine or selenocysteine residues.

  • 試験プラットフォーム
    Microplate reader

製品の特性

  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 ラベル 100 tests
    HRP Positive Control Green 1 vial
    Hydrogen Peroxide Substrate Yellow 1 x 0.1ml
    OxiRed Probe Red 1 x 0.2ml
    Peroxidase Assay Buffer WM 1 x 25ml
  • 研究分野
  • 関連性
    Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
  • 細胞内局在
    Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles.
  • 別名
    • Horseradish Peroxidase
    • HPRC1
    • HRP
    • Peroxidase
    • Peroxidase C1A
    • PRXC1A
    see all

画像

  • An example of a Fluorimetric Standard Curve
  • An example of a Colorimetric Standard Curve

プロトコール

参考文献

ab155895 has not yet been referenced specifically in any publications.

レビューと Q&A

Answer

Yes, for this assay protocol you need to use the same amount of probe irrespective of your analysis mode.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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