Anti-Peripherin 抗体 (ab4666)
Key features and details
- Rabbit polyclonal to Peripherin
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Rabbit, Cat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Peripherin antibody
Peripherin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Peripherin -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Rabbit, Cat, Human -
免疫原
Recombinant full length rat peripherin purified from E. coli.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Preservative: 0.01% Sodium azide -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab4666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| WB | 1/1000 - 1/2000. Detects a band of approximately 57 kDa. |
ターゲット情報
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機能
Class-III neuronal intermediate filament protein. -
配列類似性
Belongs to the intermediate filament family. - Information by UniProt
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参照データベース
- Entrez Gene: 5630 Human
- Entrez Gene: 19132 Mouse
- Entrez Gene: 24688 Rat
- Omim: 170710 Human
- SwissProt: P41219 Human
- SwissProt: P15331 Mouse
- SwissProt: P21807 Rat
- Unigene: 37044 Human
see all -
別名
- NEF 4 antibody
- NEF4 antibody
- Neurofilament 4 (57kD) antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody (ab4666)Immunocytochemistry/Immunofluorescence analysis of mixed neuron/glia cultures from newborn rat brain labelling Peripherin (green) with ab4666 and Neurofilament heavy polypeptide (red) with ab4680. A class of large neurons, like the one at the top right of this image, contain peripherin, while the majority of neurons and their processes contain Neurofilament heavy polypeptide and not peripherin. The blue channel reveals the localization of DNA.
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Western blot - Anti-Peripherin antibody (ab4666)Anti-Peripherin antibody (ab4666) at 1/1000 dilution + Rat spinal cord homogenate
Observed band size: 57 kDa why is the actual band size different from the predicted? -
Western blot - Anti-Peripherin antibody (ab4666)Anti-Peripherin antibody (ab4666) at 1 µg/ml +Recombinant Human Peripherin protein (ab114335) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 20 secondsSecondary antibody - goat anti-rabbit HRP preasorbed (ab97080)
プロトコール
データシートおよび資料
参考文献 (22)
ab4666 は 22 報の論文で使用されています。
- Liu W et al. Up-regulation of RNA Binding Proteins Contributes to Folate Deficiency-Induced Neural Crest Cells Dysfunction. Int J Biol Sci 16:85-98 (2020). PubMed: 31892848
- Yu H et al. Neuroprotective effects of midazolam on focal cerebral ischemia in rats through anti-apoptotic mechanisms. Int J Mol Med 43:443-451 (2019). PubMed: 30431057
- Huang CW et al. Multipotent vascular stem cells contribute to neurovascular regeneration of peripheral nerve. Stem Cell Res Ther 10:234 (2019). PubMed: 31376835
- Shen Q et al. Induction of innervation by encapsulated adipocytes with engineered vitamin A metabolism. Transl Res 192:1-14 (2018). IHC-P . PubMed: 29144959
- Salvatierra J et al. A disease mutation reveals a role for NaV1.9 in acute itch. J Clin Invest 128:5434-5447 (2018). PubMed: 30395542
