Anti-Peripherin 抗体 (ab4666)
Key features and details
- Rabbit polyclonal to Peripherin
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Peripherin antibody
Peripherin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Peripherin -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Rat, Human -
免疫原
Recombinant full length rat peripherin purified from E. coli.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Preservative: 0.01% Sodium azide -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab4666の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (2) |
Use at an assay dependent concentration.
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| WB |
1/1000 - 1/2000. Detects a band of approximately 57 kDa.
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| 特記事項 |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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WB
1/1000 - 1/2000. Detects a band of approximately 57 kDa. |
ターゲット情報
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機能
Class-III neuronal intermediate filament protein. -
配列類似性
Belongs to the intermediate filament family. - Information by UniProt
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参照データベース
- Entrez Gene: 5630 Human
- Entrez Gene: 24688 Rat
- Omim: 170710 Human
- SwissProt: P41219 Human
- SwissProt: P21807 Rat
- Unigene: 37044 Human
- Unigene: 11086 Rat
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別名
- NEF 4 antibody
- NEF4 antibody
- Neurofilament 4 (57kD) antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody (ab4666)
Immunocytochemistry/Immunofluorescence analysis of mixed neuron/glia cultures from newborn rat brain labelling Peripherin (green) with ab4666 and Neurofilament heavy polypeptide (red) with ab4680. A class of large neurons, like the one at the top right of this image, contain peripherin, while the majority of neurons and their processes contain Neurofilament heavy polypeptide and not peripherin. The blue channel reveals the localization of DNA.
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Western blot - Anti-Peripherin antibody (ab4666)Anti-Peripherin antibody (ab4666) at 1/1000 dilution + Rat spinal cord homogenate
Observed band size: 57 kDa why is the actual band size different from the predicted? -
Western blot - Anti-Peripherin antibody (ab4666)Anti-Peripherin antibody (ab4666) at 1 µg/ml +Recombinant Human Peripherin protein (ab114335) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 20 secondsSecondary antibody - goat anti-rabbit HRP preasorbed (ab97080)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (27)
ab4666 は 27 報の論文で使用されています。
- Liu W et al. Up-regulation of RNA Binding Proteins Contributes to Folate Deficiency-Induced Neural Crest Cells Dysfunction. Int J Biol Sci 16:85-98 (2020). PubMed: 31892848
- Nickolls AR et al. Transcriptional Programming of Human Mechanosensory Neuron Subtypes from Pluripotent Stem Cells. Cell Rep 30:932-946.e7 (2020). PubMed: 31968264
- Saveri P et al. Charcot-Marie-Tooth Type 2B: A New Phenotype Associated with a Novel RAB7A Mutation and Inhibited EGFR Degradation. Cells 9:N/A (2020). PubMed: 32326241
- Li J et al. CDGSH Iron Sulfur Domain 2 Deficiency Inhibits Cell Proliferation and Induces Cell Differentiation of Neuroblastoma. Pathol Oncol Res 26:1725-1733 (2020). PubMed: 31642036
- Song NN et al. Rnf220/Zc4h2-mediated monoubiquitylation of Phox2 is required for noradrenergic neuron development. Development 147:N/A (2020). PubMed: 32094113
レビューと Q&A
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