Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (spinal cord)
Specification
spinal cord
Fixative
Paraformaldehyde
Permeabilization
No
Other product details
Dilution
1/100
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS-Triton (0.3%) + azide (0.02%)
Secondary antibody
Name
Non-Abcam antibody was used: Donkey-anti-mouse Alexa 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
Additional data
Additional Notes
The tissues were fixed (animals perfused fixed) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.
We expected staining in superficial laminae of spinal cord. The range of dilution used was 1:100 to 1:3000 (1:100, 1:300, 1:1000 and 1:3000), and no staining was observed for all dilutions.
Didn't work on rat spinal cord sections.
We expected staining in superficial laminae of spinal cord. The range of dilution used was 1:100 to 1:3000 (1:100, 1:300, 1:1000 and 1:3000), and no staining was observed for all dilutions.
Didn't work on rat spinal cord sections.
Abcam response
Thank you for submitting an Abreview. ab4653 has been successfully tested in rat and IHC-Fr (PMID: 9609398), however it seems that not all protocols provide positive results. Thank you for your time, this information will surely be helpful for future customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Karine Thibault
Verified customer
投稿 Oct 17 2011