Anti-Perilipin-1 抗体 (ab3526)
Key features and details
- Rabbit polyclonal to Perilipin-1
- Suitable for: Flow Cyt, IHC-P, WB, ICC, IHC-FoFr, ICC/IF
- Reacts with: Mouse, Rat, Cow, Dog, Human, Rhesus monkey
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Perilipin-1 antibody
Perilipin-1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Perilipin-1 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt, IHC-P, WB, ICC, IHC-FoFr, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Cow, Dog, Human, Rhesus monkey -
免疫原
Synthetic peptide corresponding to Rat Perilipin-1 aa 500-600.
(Peptide available asab5009) -
ポジティブ･コントロール
- ICC: mouse 3T3-L1 cells; WB: mouse 3T3-L1 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3526の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
|
|
| IHC-P | (6) |
Use at an assay dependent concentration.
|
| WB | (1) |
1/1000 - 1/4000. Can be blocked with Perilipin-1 peptide (ab5009).
By Western blot, this antibody detects an ~62 kDa protein representing Perilipin-1 from 3T3-L1 cell extract. |
| ICC |
Use a concentration of 2 µg/ml.
|
|
| IHC-FoFr |
Use at an assay dependent concentration. PubMed: 20049087
|
|
| ICC/IF | (4) |
Use at an assay dependent concentration. PubMed: 20335253
|
| 特記事項 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
|
WB
1/1000 - 1/4000. Can be blocked with Perilipin-1 peptide (ab5009). By Western blot, this antibody detects an ~62 kDa protein representing Perilipin-1 from 3T3-L1 cell extract. |
|
ICC
Use a concentration of 2 µg/ml. |
|
IHC-FoFr
Use at an assay dependent concentration. PubMed: 20049087 |
|
ICC/IF
Use at an assay dependent concentration. PubMed: 20335253 |
ターゲット情報
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機能
Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. -
組織特異性
Adipocytes. -
配列類似性
Belongs to the perilipin family. -
翻訳後修飾
Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet. -
細胞内局在
Lipid droplet. Lipid droplet surface-associated. - Information by UniProt
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参照データベース
- Entrez Gene: 520598 Cow
- Entrez Gene: 5346 Human
- Entrez Gene: 103968 Mouse
- Entrez Gene: 25629 Rat
- Omim: 170290 Human
- SwissProt: O60240 Human
- SwissProt: Q8CGN5 Mouse
- SwissProt: P43884 Rat
see all -
別名
- Lipid droplet associated protein antibody
- Lipid droplet-associated protein antibody
- PERI antibody
see all
画像
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Immunocytochemistry - Anti-Perilipin-1 antibody (ab3526)
Immunocytochemistry analysis of 3T3-L1 cells (Mouse embryonic fibroblast cell line), 4% paraformaldehyde fixed (10 minutes) permeabilized with 0.1% Triton™ X-100 (10 minutes), and blocked with 1% BSA for (1 hour).
Perilipin A was labeled with ab3526 at 2 µg/ml in 0.1% BSA and incubated for 3 hours at room temperature with secondary antibody: Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel A: green).
Panel B: Nuclei were stained with DAPI (blue); Panel C: (red) F-actin stained with Alexa Fluor® 555 Rhodamine Phalloidin 1:300. Panel D: merged image showing cytosolic localization; Panel E: untreated; Panel F: control cells with no primary antibody.
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Western blot - Anti-Perilipin-1 antibody (ab3526)Western blot detection of Perilipin-1 in 3T3-L1 cell extract using ab3526.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (81)
ab3526 は 81 報の論文で使用されています。
- Hariri H et al. Ubiquitin specific peptidase Usp53 regulates osteoblast versus adipocyte lineage commitment. Sci Rep 11:8418 (2021). PubMed: 33875709
- Gantov M et al. Beige adipocytes contribute to breast cancer progression. Oncol Rep 45:317-328 (2021). PubMed: 33416183
- Mori H et al. The molecular and metabolic program by which white adipocytes adapt to cool physiologic temperatures. PLoS Biol 19:e3000988 (2021). PubMed: 33979328
- Liu B et al. Inhibition of Notch Signaling Promotes the Differentiation of Epicardial Progenitor Cells into Adipocytes. Stem Cells Int 2021:8859071 (2021). PubMed: 33897781
- Dong J et al. Comparison of the Therapeutic Effect of Allogeneic and Xenogeneic Small Extracellular Vesicles in Soft Tissue Repair. Int J Nanomedicine 15:6975-6991 (2020). PubMed: 33061363