Anti-Perilipin-1 抗体 (ab3526)
Key features and details
- Rabbit polyclonal to Perilipin-1
- Suitable for: WB, ICC
- Reacts with: Mouse
- Isotype: IgG
製品の概要
-
製品名
Anti-Perilipin-1 antibody
Perilipin-1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Perilipin-1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICCmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Dog
-
免疫原
Synthetic peptide corresponding to Rat Perilipin-1 aa 500-600.
(Peptide available asab5009) -
ポジティブ･コントロール
- ICC: mouse 3T3-L1 cells; WB: mouse 3T3-L1 cells
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Conjugation kits
-
Immunizing Peptide (Blocking)
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3526の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/4000. Can be blocked with Perilipin-1 peptide (ab5009).
By Western blot, this antibody detects an ~62 kDa protein representing Perilipin-1 from 3T3-L1 cell extract. |
|
| ICC |
Use a concentration of 2 µg/ml.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/4000. Can be blocked with Perilipin-1 peptide (ab5009). By Western blot, this antibody detects an ~62 kDa protein representing Perilipin-1 from 3T3-L1 cell extract. |
|
ICC
Use a concentration of 2 µg/ml. |
ターゲット情報
-
機能
Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. -
組織特異性
Adipocytes. -
配列類似性
Belongs to the perilipin family. -
翻訳後修飾
Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet. -
細胞内局在
Lipid droplet. Lipid droplet surface-associated. - Information by UniProt
-
参照データベース
- Entrez Gene: 103968 Mouse
- SwissProt: Q8CGN5 Mouse
- Unigene: 254917 Mouse
-
別名
- Lipid droplet associated protein antibody
- Lipid droplet-associated protein antibody
- PERI antibody
see all
画像
-
Immunocytochemistry - Anti-Perilipin-1 antibody (ab3526)
Immunocytochemistry analysis of 3T3-L1 cells (Mouse embryonic fibroblast cell line), 4% paraformaldehyde fixed (10 minutes) permeabilized with 0.1% Triton™ X-100 (10 minutes), and blocked with 1% BSA for (1 hour).
Perilipin A was labeled with ab3526 at 2 µg/ml in 0.1% BSA and incubated for 3 hours at room temperature with secondary antibody: Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel A: green).
Panel B: Nuclei were stained with DAPI (blue); Panel C: (red) F-actin stained with Alexa Fluor® 555 Rhodamine Phalloidin 1:300. Panel D: merged image showing cytosolic localization; Panel E: untreated; Panel F: control cells with no primary antibody.
-
Western blot - Anti-Perilipin-1 antibody (ab3526)Western blot detection of Perilipin-1 in 3T3-L1 cell extract using ab3526.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (67)
ab3526 は 67 報の論文で使用されています。
- Yang Y et al. O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity. Nat Commun 11:181 (2020). PubMed: 31924761
- Lu C et al. Long non-coding RNA ARAP1-AS1 accelerates cell proliferation and migration in breast cancer through miR-2110/HDAC2/PLIN1 axis. Biosci Rep 40:N/A (2020). PubMed: 32110804
- Rodriguez BL et al. A tissue engineering approach for repairing craniofacial volumetric muscle loss in a sheep following a 2, 4, and 6-month recovery. PLoS One 15:e0239152 (2020). PubMed: 32956427
- Kakuta T et al. Parathyroid hormone-producing cells exist in adipose tissues surrounding the parathyroid glands in hemodialysis patients with secondary hyperparathyroidism. Sci Rep 10:3290 (2020). PubMed: 32094398
- Cen S et al. TRAF4 acts as a fate checkpoint to regulate the adipogenic differentiation of MSCs by activating PKM2. EBioMedicine 54:102722 (2020). PubMed: 32268273