Anti-Pericentrin 抗体 [mAbcam 28144] - Centrosome Marker (ab28144)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [mAbcam 28144] to Pericentrin - Centrosome Marker
- Suitable for: ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker
Pericentrin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [mAbcam 28144] to Pericentrin - Centrosome Marker -
由来種
Mouse -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Chinese hamster非交差種: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- ICC/IF: HeLa cells.
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特記事項
This product has switched from a hybridoma to recombinant production method on 9th September 2020.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
mAbcam 28144 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab28144の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | (11) |
1/50.
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特記事項 |
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ICC/IF
1/50. |
ターゲット情報
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機能
Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome. -
組織特異性
Expressed in all tissues tested, including placenta, liver, kidney and thymus. -
関連疾患
Microcephalic osteodysplastic primordial dwarfism 2 -
ドメイン
Composed of a coiled-coil central region flanked by non-helical N- and C-terminals. -
細胞内局在
Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome. - Information by UniProt
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参照データベース
- Entrez Gene: 5116 Human
- Omim: 605925 Human
- SwissProt: O95613 Human
- Unigene: 474069 Human
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別名
- Centrosome Marker antibody
- Ken antibody
- Kendrin antibody
see all
画像
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Immunocytochemical analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Pericentrin with ab28144 at 1/50 dilution at 4°C overnight, followed by AlexaFluor® 488-conjugated Goat anti-Rabbit secondary
antibody (ab150077) at 1/1000 dilution at RT for 30min. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] (ab195889) was used as a counterstain at 1/200 dilution at RT for 30min. Nucleus were visualized using DAPI. The cells were 4% PFA fixed and permeabilized with 0.1% Triton X-100.Confocal image showing centrosome staining in HeLa cells.
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Immunocytochemistry/ Immunofluorescence analysis of Y79 cells labeling Pericentrin with ab28144 (red). Cells were fixed for 10 minutes at room temperature in 4% paraformaldehyde. Cells were then permeabilized using 0.2% Triton/TBS and blocked with 5% NGS in 0.1% BSA/TBS-Tween. The cells were then incubated overnight at 4°C with Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144) at 1/1000 diluted in 0.1% BSA/TBS-Tween containing 1% NGS. Cells were then stained for Hoechst (blue) and alpha-tubulin (green).
This image was generated from the hybridoma version.
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Immunocytochemistry/ Immunofluorescence analysis of A549 lung cells labeling Pericentrin with ab28144 at 1/100 dilution. Cells were fixed with formaldehyde and permeabilized with Trixton X-100. The cells were blocked with 10% serum for 30 minutes at 20°C, followed by incubation with ab28144 in PBS 10% FCS for 1 hour at 20°C. A polyclonal goat anti-mouse Alexa Fluor® 594 secondary antibody was used at 1/300 dilution.
This image was generated from the hybridoma version. -
Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Pericentrin with ab28144 at 1/200 dilution. Cells were fixed with methanol, followed by incubation with Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144) in PBS for 1 hour at 22°C. A polyclonal goat anti-mouse Cy3® secondary antibody was used at 1/200 dilution. Nuclear counterstained with DAPI.
This image was generated from the hybridoma version.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (85)
ab28144 は 85 報の論文で使用されています。
- Meyer N et al. Fetal endothelial colony-forming cell impairment after maternal kidney transplantation. Pediatr Res 93:810-817 (2023). PubMed: 35732823
- Tkach JM et al. Global cellular response to chemical perturbation of PLK4 activity and abnormal centrosome number. Elife 11:N/A (2022). PubMed: 35758262
- Chen F et al. Self-assembly of pericentriolar material in interphase cells lacking centrioles. Elife 11:N/A (2022). PubMed: 35787744
- Morretton JP et al. A catalog of numerical centrosome defects in epithelial ovarian cancers. EMBO Mol Med 14:e15670 (2022). PubMed: 36069081
- Di Paola S et al. PARP10 Mediates Mono-ADP-Ribosylation of Aurora-A Regulating G2/M Transition of the Cell Cycle. Cancers (Basel) 14:N/A (2022). PubMed: 36358629