Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse prostate and mammary epithelial)
Specification
mouse prostate and mammary epithelial
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 25°C
Fixative
try both PFA and methanol
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: alexa 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/400
Additional data
Additional Notes
antibody is not working for mouse cells (excellent for human). Abcam send me an other lot, that did not work neither. Abcam team was very nice but they cannot find me another product. So, if you plan to do mouse staining, be aware of the problem.
Abcam response
We are sorry that this antibody did not work for this customer.
The antibody was covered by the guarantee in both mouse and ICC/IF therefore the customer received first a free of charge replacement and then a credit note in compensation (since the replacement did not work either). We would be happy to receive other feedbacks from customers that used this antibody in mouse and ICC/IF. If you have any questions please contact our Scientific Support.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Aug 13 2014