Anti-Pericentrin 抗体 - Centrosome Marker (ab4448)
Key features and details
- Rabbit polyclonal to Pericentrin - Centrosome Marker
- Suitable for: ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Pericentrin antibody - Centrosome Marker
Pericentrin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Pericentrin - Centrosome Marker -
由来種
Rabbit -
特異性
This antibody should recognise both Pericentrin and Kendrin (also known as Pericentrin-2). -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, African green monkey
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免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- ICC: Human coronary artery endothelial, MCF7, HeLa and NIH/3T3 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab4448の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (27) |
Use a concentration of 0.1 - 0.5 µg/ml.
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 0.1 - 0.5 µg/ml. |
ターゲット情報
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機能
Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome. -
組織特異性
Expressed in all tissues tested, including placenta, liver, kidney and thymus. -
関連疾患
Microcephalic osteodysplastic primordial dwarfism 2 -
ドメイン
Composed of a coiled-coil central region flanked by non-helical N- and C-terminals. -
細胞内局在
Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome. - Information by UniProt
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参照データベース
- Entrez Gene: 5116 Human
- Entrez Gene: 18541 Mouse
- Omim: 605925 Human
- SwissProt: O95613 Human
- SwissProt: P48725 Mouse
- Unigene: 474069 Human
- Unigene: 251794 Mouse
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別名
- Centrosome Marker antibody
- Ken antibody
- Kendrin antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)Image courtesy of an anonymous abreview
Human coronary artery endothelial cells (HCAEC) were labeled for Pericentrin using ab4448 at 1/100 dilution in ICC/IF. Primary incubation was for 1 hour at room temperature (green). The seciondary antibody was a Goat anti-rabbit IgG AlexaFluor® conjugate.
Fixation: Formaldehyde. Permeabilisation: 0.1% Trition X-100 in HBSS.
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Immunocytochemistry - Anti-Pericentrin antibody - Centrosome Marker (ab4448)Labab4448 staining Pericentrin in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab4448 at 0.1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)Ruppenthal et al PLoS One. 2018 Jan 25;13(1):e0191734. doi: 10.1371/journal.pone.0191734. eCollection 2018. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/A representative panel of indirect immunofluorescence microscopic images shows normal (regular, n ≤ 2) and aberrant centrosome numbers (n > 2) in interphase cells.
Centrosomes were stained using anti-pericentrin antibody ab4448 (magenta), nuclear DNA is shown in blue (DAPI).
Statistical methods: Kruskal-Wallis test. Mann-Whitney U tests followed by Bonferroni-Holm p-value correction were made as post-hoc tests in order to compare the MDS and sAML patients with the control group.
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Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)This image is courtesy of Gordon Chan, University of AlbertaIF staining of pericentrin in MCF7 (Human breast adenocarcinoma cell line) cells.
The top panel is an interphase cell showing centrosome staining.
The bottom panel shows a mitotic cell with spindle pole staining.
ab4448 was used at 1/500, but also works at higher dilutions (1/1000-1/2000).
Top panel - 630X magnification; Bottom panel -1000X magnification.
The secondary antibody was Alexa-Fluor®488 anti-rabbit.
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Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)This image is courtesy of Roberto Giambruno, Marilena Ciciarello and Patrizia LaviaNIH/3T3 (Mouse embryo fibroblast cell line) cells were fixed in 100% methanol for 6 minutes at -20°C, washed 3 times in PBS then incubated with ab4448 (1/2000) for 1 hour at room temperature.
The panel of images shows the nuclei stained with DAPI (blue), ab4448 staining is shown in green. 100x magnification.
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Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)ICC/IF image of ab4448 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.
The cells were fixed in methanol (5 minutes) and incubated with the antibody (ab4448, 1 µg/ml) for 1 hour at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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Immunocytochemistry - Anti-Pericentrin antibody - Centrosome Marker (ab4448)Labab4448 staining Pericentrin in NIH3T3 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab4448 at 0.1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)ICC/IF image of ab4448 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.
The cells were fixed in 100% methanol (5 minutes) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4448, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour.
DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (381)
ab4448 は 381 報の論文で使用されています。
- Lo YH et al. Galectin-8 Is Upregulated in Keratinocytes by IL-17A and Promotes Proliferation by Regulating Mitosis in Psoriasis. J Invest Dermatol 141:503-511.e9 (2021). PubMed: 32805218
- Bosakova M et al. Mutations in GRK2 cause Jeune syndrome by impairing Hedgehog and canonical Wnt signaling. EMBO Mol Med 12:e11739 (2020). PubMed: 33200460
- Rondelet A et al. Clathrin's adaptor interaction sites are repurposed to stabilize microtubules during mitosis. J Cell Biol 219:N/A (2020). PubMed: 31932847
- Singh A et al. Centrosome amplification is a frequent event in circulating tumor cells from subjects with metastatic breast cancer. Mol Oncol 14:1898-1909 (2020). PubMed: 32255253
- Au FKC et al. Nek2-mediated GAS2L1 phosphorylation and centrosome-linker disassembly induce centrosome disjunction. J Cell Biol 219:N/A (2020). PubMed: 32289147