Anti-Pericentrin 抗体 (ab99341)
Key features and details
- Rabbit polyclonal to Pericentrin
- Suitable for: WB, IP, ICC
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Pericentrin antibody
Pericentrin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Pericentrin -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, ICCmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide, corresponding to a region within amino acids 175-225 of Human Pericentrin (NP_006022.3).
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ポジティブ･コントロール
- HeLa whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99341の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 378 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| ICC |
1/1000 - 1/5000.
Formaldehyde or Acetone fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehydefixed cells. |
| 特記事項 |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 378 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
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ICC
1/1000 - 1/5000. Formaldehyde or Acetone fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehydefixed cells. |
ターゲット情報
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機能
Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome. -
組織特異性
Expressed in all tissues tested, including placenta, liver, kidney and thymus. -
関連疾患
Microcephalic osteodysplastic primordial dwarfism 2 -
ドメイン
Composed of a coiled-coil central region flanked by non-helical N- and C-terminals. -
細胞内局在
Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome. - Information by UniProt
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参照データベース
- Entrez Gene: 5116 Human
- Omim: 605925 Human
- SwissProt: O95613 Human
- Unigene: 474069 Human
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別名
- Centrosome Marker antibody
- Ken antibody
- Kendrin antibody
see all
画像
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Immunocytochemistry - Anti-Pericentrin antibody (ab99341)Immunocytochemistry/Immunofluorescence analysis of formaldehyde-fixed asynchronous HeLa cells labelling Pericentrin with ab99341 at 1/1000 (1µg/ml). A DyLight® 594-conjugated goat anti-rabbit IgG (H+L) (1/100) was used as the secondary antibody.
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Western blot - Anti-Pericentrin antibody (ab99341)All lanes : Anti-Pericentrin antibody (ab99341) at 0.1 µg/ml
Lane 1 : HeLa cell lysate prepared using NETN lysis buffer
Lane 2 : HEK293T cell lysate prepared using NETN lysis buffer
Lysates/proteins at 50 µg per lane.
Predicted band size: 378 kDaDetection: Chemiluminescence with an exposure time of 30 seconds.
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Immunoprecipitation - Anti-Pericentrin antibody (ab99341)Detection of Pericentrin by Western Blot of Immunoprecipitates in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab99341 at 3 µg/mg lysate. Anti-Pericentrin antibody ab99342, which recognizes a downstream epitope, was used at 1 µg/ml for subsequent WB detection.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab99341 は論文での使用が確認できていません。
