Key features and details
- PerCP/Cy5.5® Mouse monoclonal [29E.2A3] to PD-L1
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
- Isotype: IgG2a
製品名PerCP/Cy5.5® Anti-PD-L1 antibody [29E.2A3]
PD-L1 一次抗体 製品一覧
製品の詳細PerCP/Cy5.5® Mouse monoclonal [29E.2A3] to PD-L1
標識PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
Tested Applications & Species
Application Species Flow CytHuman
Full length native protein (purified) corresponding to Human PD-L1.
Database link: Q9NZQ7
- Flow cyt: peripheral blood mononuclear cells.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. Do Not Freeze.
Preservative: 0.1% Sodium azide
Concentration information loading...
特記事項（精製）Purified from TCS.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Use 4µg for 10 cells.
Use 4µg for 10 cells.
機能Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
組織特異性Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
配列類似性Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
細胞内局在Cell membrane and Endomembrane system.
- Information by UniProt
- B7 H antibody
- B7 H1 antibody
- B7 homolog 1 antibody
Separation of human monocytes (Red) from cellular debris (black-dashed) in flow cytometric analysis labeling CD274 using ab272349. (4 μl reagent per milion cells in 100 μl of cell suspension).
Flow cytometric analysis of peripheral blood mononuclear cells labeling CD274 with ab272349 at 4μl reagent per milion cells in 100 μl of cell suspension. (4 μl reagent per milion cells in 100 μl of cell suspension).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.