PerCP/Cy5.5® Anti-PD-L1 抗体 [29E.2A3] (ab272349)
![Flow Cytometry - PerCP/Cy5.5® Anti-PD-L1 antibody [29E.2A3] (ab272349)](https://www.abcam.co.jp/ps/products/272/ab272349/Images/ab272349-387926-AntiPDL1-antibody-29E2A3-flowcytometry-monocytes-human.jpg "Flow Cytometry - PerCP/Cy5.5® Anti-PD-L1 antibody [29E.2A3] (ab272349)")
Key features and details
- PerCP/Cy5.5® Mouse monoclonal [29E.2A3] to PD-L1
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
- Isotype: IgG2a
製品の概要
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製品名
PerCP/Cy5.5® Anti-PD-L1 antibody [29E.2A3]
PD-L1 一次抗体 製品一覧 -
製品の詳細
PerCP/Cy5.5® Mouse monoclonal [29E.2A3] to PD-L1 -
由来種
Mouse -
標識
PerCP/Cy5.5®. Ex: 482nm, Em: 690nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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免疫原
Full length native protein (purified) corresponding to Human PD-L1.
Database link: Q9NZQ7 -
ポジティブ･コントロール
- Flow cyt: peripheral blood mononuclear cells.
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特記事項
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Size exclusion -
特記事項（精製）
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
29E.2A3 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272349 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| Flow Cyt |
Human
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use 4µg for 10 cells.
|
| 特記事項 |
|---|
|
Flow Cyt
Use 4µg for 10 cells. |
ターゲット情報
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機能
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. -
組織特異性
Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. -
配列類似性
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Cell membrane and Endomembrane system. - Information by UniProt
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参照データベース
- Entrez Gene: 29126 Human
- Omim: 605402 Human
- SwissProt: Q9NZQ7 Human
- Unigene: 521989 Human
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別名
- B7 H antibody
- B7 H1 antibody
- B7 homolog 1 antibody
see all
画像
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Flow Cytometry - PerCP/Cy5.5® Anti-PD-L1 antibody [29E.2A3] (ab272349)
Separation of human monocytes (Red) from cellular debris (black-dashed) in flow cytometric analysis labeling CD274 using ab272349. (4 μl reagent per milion cells in 100 μl of cell suspension).
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![Flow Cytometry - PerCP/Cy5.5® Anti-PD-L1 antibody [29E.2A3] (ab272349)](https://www.abcam.co.jp/ps/products/272/ab272349/Images/ab272349-387923-AntiPDL1-antibody-29E2A3-flowcytometry-peripheral-blood-mononuclear-cells-human.jpg)
Flow Cytometry - PerCP/Cy5.5® Anti-PD-L1 antibody [29E.2A3] (ab272349)Flow cytometric analysis of peripheral blood mononuclear cells labeling CD274 with ab272349 at 4μl reagent per milion cells in 100 μl of cell suspension. (4 μl reagent per milion cells in 100 μl of cell suspension).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab272349 は論文での使用が確認できていません。