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Anti-PER2 (phospho S662) 抗体 [EPR19820] - BSA and Azide free (ab271972)

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Dot Blot - Anti-PER2 antibody [EPR19820] (ab271972)
  • Anti-PER2 (phospho S662) antibody [EPR19820] - BSA and Azide free (ab271972)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19820] to PER2 (phospho S662) - BSA and Azide free
  • Suitable for: WB, Dot blot
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-PER2 (phospho S662) antibody [EPR19820] - BSA and Azide free
    PER2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR19820] to PER2 (phospho S662) - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, Dot blotmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HEK-293T whole cell lysate transfected with hPER2.

  • 特記事項

    ab271972 is the carrier-free version of ab206377. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab271972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 137 kDa.
Dot blot
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 137 kDa.
Dot blot
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK
    NPAS2-BMAL1
    BMAL2-induced transactivation.

  • 組織特異性

    Widely expressed. Found in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. High levels in skeletal muscle and pancreas. Low level in lung.

  • 関連疾患

    Defects in PER2 are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms.

  • 配列類似性

    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.

  • 翻訳後修飾

    Phosphorylated by CSNK1E and CSNK1D. Phosphorylation results in PER2 protein degradation.

  • 細胞内局在

    Nucleus. Cytoplasm. Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Circadian clock protein PERIOD 2 antibody
      • FASPS antibody
      • FASPS1 antibody
      • hPER 2 antibody
      • hPER2 antibody
      • KIAA0347 antibody
      • OTTHUMP00000164476 antibody
      • PER 2 antibody
      • PER2 antibody
      • PER2_HUMAN antibody
      • Period 2 antibody
      • Period 2 isoform 1 antibody
      • Period circadian clock 2 antibody
      • Period circadian protein 2 antibody
      • Period circadian protein homolog 2 antibody
      • Period homolog 2 (Drosophila) antibody
      • Period homolog 2 antibody
      • Period, Drosophila, homolog of, 2 antibody
      • Period2 antibody
      see all

    画像

    • Dot Blot - Anti-PER2 antibody [EPR19820] (ab271972)
      Dot Blot - Anti-PER2 antibody [EPR19820] (ab271972)

      Lane 1: PER2 (phospho S662) peptide labelled with ab206377 primary antibody at 1/1000 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100000 dilution.

      Lane 2: PER2 non-phospho peptide labelled with ab206377 primary antibody at 1/1000 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100000 dilution.

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206377).
    • Anti-PER2 (phospho S662) antibody [EPR19820] - BSA and Azide free (ab271972)
      Anti-PER2 (phospho S662) antibody [EPR19820] - BSA and Azide free (ab271972)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab271972 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab271972 は論文での使用が確認できていません。

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