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Anti-Peptide YY/PYY 抗体 (ab22663)

  • Datasheet
Reviews (5)Q&A (4)References (16)

Product price, shipping and contact information

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Abpromise

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (ab22663)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (ab22663)
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Peptide YY/PYY antibody (ab22663)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (ab22663)

Key features and details

  • Rabbit polyclonal to Peptide YY/PYY
  • Suitable for: IHC-FoFr, IHC-P
  • Reacts with: Rat
  • Isotype: IgG

こちらの製品もご検討ください

一次抗体
Product image
Anti-GLP-1 antibody [EPR4042-1] (ab108443)
タンパク質
Product image
Recombinant Human Peptide YY/PYY protein (ab114446)
ELISA
Product image
Human GLP1 (7-36) ELISA Kit  (ab184857)

関連製品

製品の概要

  • 製品名

    Anti-Peptide YY/PYY antibody
    Peptide YY/PYY 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Peptide YY/PYY
  • 由来種

    Rabbit
  • 特異性

    Immunostaining may be abolished by preincubation with 1nmol synthetic porcine PYY per mL diluted antibody. NPY and PP (10nmol/mL) have no effect on immunostaining.
  • アプリケーション

    適用あり: IHC-FoFr, IHC-Pmore details
  • 種交差性

    交差種: Rat
    交差が予測される動物種: Rabbit, Dog, Human
  • 免疫原

    Full length native protein (purified) corresponding to Pig Peptide YY/PYY aa 1-36.
    Sequence:

    YPAKPEAPGE DASPEELSRY YASLRHYLNL VTRQRY


    Database link: P68005
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    Preservative: 0.07% Sodium azide
  • Concentration information loading...
  • 精製度

    Whole antiserum
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Neurotransmitter
    • Neuropeptides
    • Hormones
    • Metabolism
    • Types of disease
    • Obesity

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Peptide YY/PYY protein (ab114446)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab22663の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-FoFr
Use at an assay dependent concentration.
IHC-P (4)
Use at an assay dependent concentration.
特記事項
IHC-FoFr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility.
  • 配列類似性

    Belongs to the NPY family.
  • 細胞内局在

    Secreted.
  • Target information above from: UniProt accession P10082 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 607156 Dog
    • Entrez Gene: 5697 Human
    • Entrez Gene: 287730 Rat
    • Omim: 600781 Human
    • SwissProt: P68004 Dog
    • SwissProt: P10082 Human
    • SwissProt: Q9TR93 Rabbit
    • SwissProt: P10631 Rat
    • Unigene: 169249 Human
    see all
  • 別名

    • GHYY antibody
    • MGC19143 antibody
    • Peptide tyrosine tyrosine antibody
    • peptide YY antibody
    • Peptide YY like antibody
    • Peptide YY precursor antibody
    • Peptide YY(3-36) antibody
    • Prepro PYY antibody
    • PYY 1 antibody
    • PYY antibody
    • PYY II antibody
    • PYY-I antibody
    • PYY-II antibody
    • PYY_HUMAN antibody
    • PYY1 antibody
    • RATGHYY antibody
    • Yy antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (ab22663)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (ab22663)Image courtesy of Carl Hobbs, Kings College London, U.K.

    IHC image of Peptide YY/PYY staining in mouse colon formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/9000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (ab22663)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (ab22663)Image courtesy of Carl Hobbs, Kings College London, U.K.

    IHC image of Peptide YY/PYY staining in rat pancreas formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/9000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.

    See Abreview

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Peptide YY/PYY antibody (ab22663)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Peptide YY/PYY antibody (ab22663)
    Adjacent de-waxed sections of Bouin-fixed fetal (14 day) rat pancreas stained with rabbit polyclonal antisera to PYY (ab22663; panel a) and glucagon (ab18461; panel b). Arrows indicate identical cell profiles. From Ali-Rachedi et al, 1984.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (ab22663)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peptide YY/PYY antibody (ab22663)Image from Acosta A et al., PLoS One. 2011;6(10):e26137. Epub 2011 Oct 10. Fig 2.; doi:10.1371/journal.pone.0026137; October 10, 2011, PLoS ONE 6(10): e26137.

    ab22663 staining Peptide YY/PYY (green) in murine pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Tissue (left, WT; right, Peptide YY/PYY KO) was fixed with paraformaldehyde and blocked with 3% H2O2 in methanol, followed by antigen retrieval with trypsin. Samples were incubated with primary antibody (1/2000 in TNT) overnight. An AlexaFluor®488-conjugated donkey anti-rabbit IgG (1/1000 in TNT) was used as the secondary antibody. Nuclei were stained with DAPI (red).

プロトコール

  • Immunohistochemistry protocols

Click here to view the general protocols

データシートおよび資料

  • Datasheet download

    Download

参考文献 (16)

ab22663 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab22663 は 16 報の論文で使用されています。

  • Ichijo R  et al. Vasculature-driven stem cell population coordinates tissue scaling in dynamic organs. Sci Adv 7:N/A (2021). PubMed: 33568475
  • Camacho-Ramírez A  et al. The Leading Role of Peptide Tyrosine Tyrosine in Glycemic Control After Roux-en-Y Gastric Bypass in Rats. Obes Surg 30:697-706 (2020). PubMed: 31701411
  • Wang H  et al. Expression patterns of L-amino acid receptors in the murine STC-1 enteroendocrine cell line. Cell Tissue Res 378:471-483 (2019). PubMed: 31410629
  • Guida C  et al. PYY plays a key role in the resolution of diabetes following bariatric surgery in humans. EBioMedicine 40:67-76 (2019). PubMed: 30639417
  • Lu VB  et al. Adenosine triphosphate is co-secreted with glucagon-like peptide-1 to modulate intestinal enterocytes and afferent neurons. Nat Commun 10:1029 (2019). PubMed: 30833673
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-9 of 9 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Peptide YY/PYY antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Colon
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Read More

MR. Carl Hobbs

Verified customer

投稿 Dec 16 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Peptide YY/PYY antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: HIER
Permeabilization
No
Specification
Pancreas
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Read More

MR. Carl Hobbs

Verified customer

投稿 Dec 16 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Peptide YY/PYY antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Common marmoset Tissue sections (Jejunum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Jejunum
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Read More

MR. Carl Hobbs

Verified customer

投稿 Dec 13 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Peptide YY / PYY antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
Pancreas
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

DR. Srividya Vasu

Verified customer

投稿 Oct 08 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-Peptide YY/PYY antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (small intestine)
Permeabilization
Yes - 0.1% TX-100 in AB-solution
Specification
small intestine
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Acetone
Read More

Dr. Andreas Gille

Verified customer

投稿 Dec 05 2007

Question

I have placed an order of ab22663 without the code you kindly provided. I will let you know whether the anti-PYY reacts with human cell line. I need the blocking peptide for negative control. Would you please have the information to me soon.

Read More

Abcam community

Verified customer

Asked on May 01 2012

Answer

Thank you for your reply. After following up with the lab, unfortunately we will not be able to add the immunogenic peptide for ab22663 to our catalog. It is possible that you may be able to have the peptide custom synthesized from an outside company using the sequence: YPAKPEAPGE DASPEELSRY YASLRHYLNL VTRQRY. I am sorry I could not be more helpful, please let me know if you need any additional information or assistance.

Read More

Abcam Scientific Support

Answered on May 01 2012

Question

I would like to test this Peptide YY antibody for use on human samples.

Read More

Abcam community

Verified customer

Asked on Apr 23 2012

Answer



DISCOUNT CODE: ***
Expiration date: August 24th, 2012

I am very pleased to hear you would like to accept our offer and test ab22663 in human. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forhuman and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Abcam Scientific Support

Answered on Apr 23 2012

Question

I have recently ordered 2 vials of ab22663. They were supposed to contain 50ul each but only contained 40ul.

Read More

Abcam community

Verified customer

Asked on Dec 22 2011

Answer

I am sorry this product did not contain the expected volume and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab22663. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

Read More

Abcam Scientific Support

Answered on Dec 22 2011

Question

I am getting unexpected staining of rat ileum sections using ab22663. I expect this antibody to stain cells at the base of the villi.

Read More

Abcam community

Verified customer

Asked on Dec 15 2011

Answer

Thank you for your enquiry. As we discussed, please forward along a representative image of your IHC staining, along with brief descriptions of sample preparation and protocols used. Thanks in advance for the additional information!

Read More

Abcam Scientific Support

Answered on Dec 15 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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