製品の概要

  • 製品名

    Pepsin Solution (Antigen Retrieval)
  • アプリケーション

    適用あり: IHC-Pmore details
  • 特記事項

    Pepsin for protease-induced antigen retrieval (PIER) during IHC procedures (ab64201). Ready-to-use formulation. Tris buffer pH 2.0

    Staining Protocol:

    1. Incubate tissue section for 10 minutes at 37°C.
    2. Rinse and proceed with immunostaining.

     Pepsin is a commonly used digestive enzyme for immunohistochemical procedures.

    Other products for IHC

    Other enzymes for antigen retrieval include: Proteinase K Antigen Retrieval Solution ab64220 and Trypsin Antigen Retrieval Kit ab259256.

    Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab64201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.

画像

  • ab64201 Pepsin Solution used with Laminin on Human Kidney

参考文献

ab64201 has not yet been referenced specifically in any publications.

レビューと Q&A

1-4 of 4 Abreviews or Q&A

Answer

Thank you for your inquiry.

I assume your question is regarding ab64201 (Pepsin Solution)?

Unfortunately, ab64201 is of porcine origin not of human.

We also do not have any data about this product that tested any other application than pretreatment of tissue for IHC.

I am sorry to confirm that we do not have any human pepsin in our catalog.

I hope this information was nevertheless helpful and wish you good luck in your research.

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Answer

Thank you for contacting us.
I am sorry to hear that the antibodie might not be working as expected.

As we discussed over the phone the following troubleshooting tips might help in finding the cause of the problem:

1) Try antigen retrieval (enzymatic) using one of the products we discussed (ab64201, ab64220, ab64205 or ab970).

2) Check if secondary antibody works (e.g. with fluorescent plate reader or fluorescence micrscope, or with another primary Ab)

Please let me know if and how your results improving.
I wish you good luck and look forward to hear back from you.

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Answer



Unfortunately we do not have any experimental data regarding using the Pepsin Solution ab64201 for RNA-FISH, we do not control the activity of the pepsin and we do not know if it is RNAse-free.

Please note that the concentration of pepsin in ab64201 is 0.25%, not 20% as currently stated on the product datasheet. This information will be corrected shortly.

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Answer

Gracias por contactar con Abcam.



La concentración de este producto es 0,25%.



Espero que esta información sea útil a usted. Por favor, háganos saber si usted tiene cualquier otra pregunta.

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