Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

PE Anti-TLR4 抗体 [HTA125] (ab11227)

Submit a review Q&A (2)
Flow Cytometry - PE Anti-TLR4 antibody [HTA125] (ab11227)
  • Flow Cytometry - PE Anti-TLR4 antibody [HTA125] (ab11227)

Key features and details

  • PE Mouse monoclonal [HTA125] to TLR4
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Isotype: IgG2a

Conjugates logo Related conjugates and formulations

Carrier Free Unconjugated

製品の概要

  • 製品名

    PE Anti-TLR4 antibody [HTA125]
    TLR4 一次抗体 製品一覧

  • 製品の詳細

    PE Mouse monoclonal [HTA125] to TLR4

  • 由来種

    Mouse

  • 標識

    PE. Ex: 488nm, Em: 575nm

  • アプリケーション

    適用あり: Flow Cytmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Tissue, cells or virus corresponding to Human TLR4.

  • ポジティブ･コントロール

    • Human monocytes and macrophages/whole blood.

  • 特記事項

    This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

    TLR4 expression on U937 cells can be upregulated following stimulation with e.g. LPS or IFN gamma.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab11227の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
1/1.

Use 10 µl of undiluted antibody to label 106 cells or 100 µl whole blood.

ab91363 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
特記事項
Flow Cyt
1/1.

Use 10 µl of undiluted antibody to label 106 cells or 100 µl whole blood.

ab91363 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.

  • 組織特異性

    Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.

  • 関連疾患

    Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.

  • 配列類似性

    Belongs to the Toll-like receptor family.
    Contains 18 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.

  • ドメイン

    The TIR domain mediates interaction with NOX4.

  • 翻訳後修飾

    N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ARMD10 antibody
      • CD284 antibody
      • CD284 antigen antibody
      • Homolog of Drosophila toll antibody
      • hToll antibody
      • TLR 4 antibody
      • TLR4 antibody
      • TLR4_HUMAN antibody
      • TOLL antibody
      • Toll like receptor 4 antibody
      • Toll-like receptor 4 antibody
      see all

    画像

    • Flow Cytometry - PE Anti-TLR4 antibody [HTA125] (ab11227)
      Flow Cytometry - PE Anti-TLR4 antibody [HTA125] (ab11227)
      Staining of U937 cells with ab11227.
    • Flow Cytometry - PE Anti-TLR4 antibody [HTA125] (ab11227)
      Flow Cytometry - PE Anti-TLR4 antibody [HTA125] (ab11227)
      Ab11227 staining on human peripheral blood monocytes.

    参考文献 (0)

    ab11227 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab11227 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    Could you please tell me the concentration of AB11227, Lot 142704. In addition could you please let me know which dilution of this antibody you recommend for flow cytometry?

    Read More
    Answer

    Thank you for your email. We are unable to measure the concentration of our PE conjugates, so I cannot give you precise details. However, the concentration of the antibody will be approximately 0.1mg/ml. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    Can you tell me the percentage of monocytes that express TLR4 using this antibody in FACS on live cells? Can you provide me with a figure comparing a plot using the antibody versus an isotype control?

    Read More
    Answer

    Thank you for your enquiry. Expression of TLR4 on normal resting monocytes is low and for this reason ab11227 is routinely QC'd on the U937 cell line which demonstrates a reasonable level of expression. I can attach an image here so I will send you a separate email with some histograms. However, I am pleased to say that I was able to obtain a histogram showing our current batch of ab11227 tested on monocytes. Expression of TLR4 is low, overlapping the isotype control, but still distinct. I believe that TLR4 expression on this cell type can be upregulated following stimulation with e.g. LPS or IFN gamma. You could refer to the literature for further information but the following paper will provide a good starting point. Bosisio, D. et al. (2002). Stimulation of toll-like receptor 4 expression in human mononuclear phagocytes by interferon-gamma: a molecular basis for priming and synergism with bacterial lipopolysaccharide. Blood. 99(9):3427-3431. Link to full text: http://www.bloodjournal.org/cgi/reprint/99/9/3427 I will send you a separate email with the histograms. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com