Key features and details
- PE Mouse monoclonal [mN1A] to Notch 1
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
Related conjugates and formulations
製品名PE Anti-Notch 1 antibody [mN1A]
Notch 1 一次抗体 製品一覧
製品の詳細PE Mouse monoclonal [mN1A] to Notch 1
標識PE. Ex: 488nm, Em: 575nm
特異性ab93569 reacts with the intracellular domain of Notch1, but not with Notch2, 3, or 4. The antibody has a low affinity for the full length (unprocessed or heterodimeric cell surface) forms of Notch1.
アプリケーション適用あり: Flow Cytmore details
Recombinant fragment corresponding to Mouse Notch1.
Database link: Q01705
- C57Bl/6 Mouse thymocytes
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保存方法Shipped at 4°C. Store at +4°C.
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, PBS
Concentration information loading...
精製度Protein G purified
The Abpromise guarantee
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia.
組織特異性In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.
関連疾患Defects in NOTCH1 are a cause of bicuspid aortic valve (BAV) [MIM:109730]. A common defect in the aortic valve in which two rather than three leaflets are present. It is often associated with aortic valve calcification and insufficiency. In extreme cases, the blood flow may be so restricted that the left ventricle fails to grow, resulting in hypoplastic left heart syndrome.
配列類似性Belongs to the NOTCH family.
Contains 5 ANK repeats.
Contains 36 EGF-like domains.
Contains 3 LNR (Lin/Notch) repeats.
翻訳後修飾Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
O-glycosylated on the EGF-like domains. Contains both O-linked fucose and O-linked glucose.
Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH.
細胞内局在Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
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