PE Anti-LAG-3 抗体 [EPR20261] (ab225484)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR20261] to LAG-3
- Suitable for: Flow Cyt, Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
製品の概要
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製品名
PE Anti-LAG-3 antibody [EPR20261]
LAG-3 一次抗体 製品一覧 -
製品の詳細
PE Rabbit monoclonal [EPR20261] to LAG-3 -
由来種
Rabbit -
標識
PE. Ex: 488nm, Em: 575nm -
アプリケーション
適用あり: Flow Cyt, Flow Cyt (Intra)more details -
種交差性
交差種: Human -
免疫原
Recombinant fragment within Human LAG-3 aa 1-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P18627 -
ポジティブ・コントロール
- Flow Cyt: A431 cells and HEK-293T cells transfected with a GFP-tagged human LAG3 construct.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Store In the Dark. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR20261 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Anti-LAG-3 antibody [EPR20261] (ab209236)
- Alexa Fluor® 488 Anti-LAG-3 antibody [EPR20261] (ab225485)
- Alexa Fluor® 647 Anti-LAG-3 antibody [EPR20261] (ab225486)
- Anti-LAG-3 antibody [EPR20261] - BSA and Azide free (ab227579)
- Alexa Fluor® 555 Anti-LAG-3 antibody [EPR20261] (ab280858)
- Anti-LAG-3 antibody [EPR20261] - Low endotoxin, Azide free (ab282638)
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab225484の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
1/1000.
The cellular localisation of this product has been verified in ICC/IF. |
|
Flow Cyt (Intra) |
1/1000.
|
特記事項 |
---|
Flow Cyt
1/1000. The cellular localisation of this product has been verified in ICC/IF. |
Flow Cyt (Intra)
1/1000. |
ターゲット情報
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機能
Involved in lymphocyte activation. Binds to HLA class-II antigens. -
組織特異性
On cell surface of activated NK and T-lymphocytes. -
配列類似性
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3902 Human
- Omim: 153337 Human
- SwissProt: P18627 Human
- Unigene: 409523 Human
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別名
- CD223 antibody
- CD223 antigen antibody
- FDC protein antibody
see all
画像
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Flow cytometric analysis of HEK-293T (Human epithelial cell line from embryonic kidney) cells transfected with a GFP-tagged human LAG3 construct labelling LAG-3 with ab225484 at 1/50 dilution.
Note: Fresh cells without fixation and permeabilization were used to perform FC testing. Only GFP positive population results in LAG3 positive staining (Q2).
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Overlay histogram showing A431 cells stained with ab225284 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225484, 1/1000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab225484 は論文での使用が確認できていません。