PE Anti-CD8 alpha 抗体 [53-6.7] (ab272343)

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Flow Cytometry - PE Anti-CD8 alpha antibody [53-6.7] (ab272343)
  • Flow Cytometry - PE Anti-CD8 alpha antibody [53-6.7] (ab272343)

Key features and details

  • PE Rat monoclonal [53-6.7] to CD8 alpha
  • Suitable for: Flow Cyt
  • Reacts with: Mouse
  • Conjugation: PE. Ex: 488nm, Em: 575nm
  • Isotype: IgG2a

製品の概要

  • 製品名

    PE Anti-CD8 alpha antibody [53-6.7]
    CD8 alpha 一次抗体 製品一覧

  • 製品の詳細

    PE Rat monoclonal [53-6.7] to CD8 alpha

  • 由来種

    Rat

  • 標識

    PE. Ex: 488nm, Em: 575nm

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    See all applications and species data

  • 免疫原

    Tissue, cells or virus corresponding to Mouse CD8 alpha. Murine spleen cells.

  • エピトープ

    Extracellular

  • ポジティブ･コントロール

    • Flow Cyt: Murine splenocyte suspension.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Mouse
アプリケーション Abreviews 特記事項
Flow Cyt
Use a concentration of 2 µg/ml.
特記事項
Flow Cyt
Use a concentration of 2 µg/ml.

ターゲット情報

  • 機能

    Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

  • 関連疾患

    Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.

  • 配列類似性

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • 翻訳後修飾

    All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.

  • 細胞内局在

    Secreted and Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • alpha polypeptide (p32) antibody
      • CD_antigen=CD8a antibody
      • CD8 antibody
      • CD8 antigen alpha polypeptide antibody
      • CD8 antigen alpha polypeptide (p32) antibody
      • CD8 antigen, alpha polypeptide (p32) antibody
      • CD8a antibody
      • CD8A antigen antibody
      • CD8A molecule antibody
      • CD8A_HUMAN antibody
      • Leu2 antibody
      • Leu2 T lymphocyte antigen antibody
      • Ly 2 antibody
      • Ly 35 antibody
      • Ly B antibody
      • Ly2 antibody
      • Ly3 antibody
      • Ly35 antibody
      • LyB antibody
      • Lyt 2.1 lymphocyte differentiation antigen (AA at 100) antibody
      • LYT3 antibody
      • MAL antibody
      • OKT8 T cell antigen antibody
      • OTTHUMP00000160760 antibody
      • OTTHUMP00000160764 antibody
      • OTTHUMP00000203528 antibody
      • OTTHUMP00000203721 antibody
      • p32 antibody
      • T cell antigen Leu2 antibody
      • T cell co receptor antibody
      • T lymphocyte differentiation antigen T8/Leu 2 antibody
      • T-cell surface glycoprotein CD8 alpha chain antibody
      • T-cell surface glycoprotein Lyt 2 antibody
      • T-lymphocyte differentiation antigen T8/Leu-2 antibody
      • T8 T cell antigen antibody
      • T8/Leu-2 T-lymphocyte differentiation antigen antibody
      see all

    画像

    • Flow Cytometry - PE Anti-CD8 alpha antibody [53-6.7] (ab272343)
      Flow Cytometry - PE Anti-CD8 alpha antibody [53-6.7] (ab272343)

      Flow cytometry surface staining pattern of murine splenocyte suspension stained using ab272343 at 1.67 μg/ml.

    • Flow Cytometry - PE Anti-CD8 alpha antibody [53-6.7] (ab272343)
      Flow Cytometry - PE Anti-CD8 alpha antibody [53-6.7] (ab272343)

      Separation of murine CD8a positive cells (red-filled) from murine CD8a negative cells (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using ab272343 at 1.67 μg/ml.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab272343 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab272343 は論文での使用が確認できていません。

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