Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Thymus)
Specification
Thymus
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer PH6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Other product details
Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: in blocking buffer
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
positive control using anti-CD4 antibody from other maker was included in this staining.
Secondary antibody was not used since the primary antibody was conjugated with phycoerythrin.
Secondary antibody was not used since the primary antibody was conjugated with phycoerythrin.
Abcam response
Thanks for your honest feedback. This antibody has not previously been tested in IHC-P or for reactivity to human CD4. It may be that ab18282 is either not reactive to human samples or is not useful for IHC-P. This is certainly valuable information that will be helpful for other researchers. Thanks again for submitting your review!
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ruifeng Teng
Verified customer
投稿 Apr 30 2012