PE Anti-CD22 抗体 [RFB4] (ab23620)
Key features and details
- PE Mouse monoclonal [RFB4] to CD22
- Suitable for: IHC-Fr, Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
製品の概要
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製品名
PE Anti-CD22 antibody [RFB4]
CD22 一次抗体 製品一覧 -
製品の詳細
PE Mouse monoclonal [RFB4] to CD22 -
由来種
Mouse -
標識
PE. Ex: 488nm, Em: 575nm -
特異性
CD22 is in principle a B lineage antigen, that is present in the cytoplasm of progenitor B-cells and on the membrane of the majority of mature peripheral B-lymphocytes. Furthermore, it is strongly expressed on Hairy Cell Leukemia cells and very weakly on some other leukemias. Antigen distribution: Peripheral blood lymphocytes 12±3% T-cells (E+) < 1% B-cells (E-, Ig+) 92±3% Monocytes (CD14+) < 1% Granulocytes < 1% Thymocytes < 1% -
アプリケーション
適用あり: IHC-Fr, Flow Cytmore details
適用なし: IHC-P -
種交差性
交差種: Human -
免疫原
Purified tonsil lymphocytes (Human).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.3
Preservative: 0.1% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
特記事項（精製）
Purification by IEP (immunoelectrophoresis). -
一次抗体 備考
ab23620 can be used for: - Quantitative determination of peripheral B-cells (CD22+) in blood. - Identification of CD22+-cells in tissue sections. - Determination of B-cell origin of lymphoid neoplasms. - Quantitative elimination or isolation of B-cells by flow cytometry or magnet beads. -
ポリ/モノ
モノクローナル -
クローン名
RFB4 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab23620の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
|
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| Flow Cyt |
Use at an assay dependent concentration.
Use maximum of 5ul for 10^6 cells. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. Use maximum of 5ul for 10^6 cells. ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
追加情報
Is unsuitable for IHC-P.
ターゲット情報
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機能
Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. -
組織特異性
B-lymphocytes. -
配列類似性
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
ドメイン
Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
翻訳後修飾
Phosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.
Phosphorylated on tyrosine residues by LYN. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 933 Human
- Omim: 107266 Human
- SwissProt: P20273 Human
- Unigene: 579691 Human
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別名
- B cell receptor CD22 precursor antibody
- B lymphocyte cell adhesion molecule antibody
- B-cell receptor CD22 antibody
see all
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab23620 は 2 報の論文で使用されています。
- Ito D et al. A double blinded, placebo-controlled pilot study to examine reduction of CD34 +/CD117 +/CD133 + lymphoma progenitor cells and duration of remission induced by neoadjuvant valspodar in dogs with large B-cell lymphoma. F1000Res 4:42 (2015). PubMed: 28357033
- Ito D et al. Exclusion of cytoplasmic fragments in flow cytometric analysis of lymph node samples from dogs with lymphoma using membrane-permeable violet laser-excitable DNA-binding fluorescent dye (DyeCycle Violet). Vet Clin Pathol 39:494-8 (2010). PubMed: 21198735