Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7187] to PDLIM1/CLP36 - BSA and Azide free
- Suitable for: ICC, WB
- Reacts with: Mouse, Rat, Human
製品名Anti-PDLIM1/CLP36 antibody [EPR7187] - BSA and Azide free
PDLIM1/CLP36 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR7187] to PDLIM1/CLP36 - BSA and Azide free
アプリケーション適用あり: ICC, WBmore details
適用なし: IHC-P or IP
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human PDLIM1/CLP36 aa 200-300. The exact sequence is proprietary.
Database link: O00151
ab248135 is the carrier-free version of ab126628 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248135 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as PDLIM1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
Concentration information loading...
Our Abpromise guarantee covers the use of ab248135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.|
機能Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton.
組織特異性Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes.
配列類似性Contains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain.
細胞内局在Cytoplasm. Cytoplasm > cytoskeleton. Associates with the actin stress fibers.
- Information by UniProt
- C terminal LIM domain protein 1 antibody
- C-terminal LIM domain protein 1 antibody
- Carboxy terminal LIM domain protein 1 antibody
All lanes : Anti-PDLIM1/CLP36 antibody [EPR7187] (ab126628) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Caco 2 cell lysate
Lane 5 : Saos 2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 36 kDa
This data was developed using ab126628, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.