Anti-PDIA6 抗体 [EPR10132(B)] (ab154820)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10132(B)] to PDIA6
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PDIA6 antibody [EPR10132(B)]
PDIA6 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR10132(B)] to PDIA6 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, ICC/IFmore details -
種交差性
交差種: Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HepG2, K-562, HT-1080 cell lysates. Rat spleen, Rat testis, and Rat liver tissue lysates. Flow Cyt (Intra): HT-1080 cells. ICC/IF: K-562 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR10132(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154820の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
|
ICC/IF | (1) |
1/100.
For unpurified use at 1/250 - 1/500. |
特記事項 |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 48 kDa. |
ICC/IF
1/100. For unpurified use at 1/250 - 1/500. |
ターゲット情報
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機能
May function as a chaperone that inhibits aggregation of misfolded proteins. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin. -
組織特異性
Expressed in platelets (at protein level). -
配列類似性
Belongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains. -
細胞内局在
Endoplasmic reticulum lumen. Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 10130 Human
- Entrez Gene: 286906 Rat
- Omim: 611099 Human
- SwissProt: Q15084 Human
- SwissProt: Q63081 Rat
- Unigene: 212102 Human
- Unigene: 580464 Human
- Unigene: 2685 Rat
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別名
- Endoplasmic reticulum protein 5 antibody
- ER protein 5 antibody
- ERp5 antibody
see all
画像
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Lanes 1-3 : Anti-PDIA6 antibody [EPR10132(B)] (ab154820) at 1/1000 dilution (Purified)
Lanes 4-6 : Anti-PDIA6 antibody [EPR10132(B)] (ab154820) at 1/1000 dilution
Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 3 : HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate
Lane 4 : Rat spleen lysate
Lane 5 : Rat testis lysate
Lane 6 : Rat liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa -
Flow Cytometry analysis of HT-1080 (Human fibrosarcoma epithelial cell) cells labelling PDIA6 with purified ab154820 at 1/100 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150081) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (blue).
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Immunocytochemistry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling PDIA6 with purified ab154820 at 1:100 dilution (9.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as a nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab154820 は 4 報の論文で使用されています。
- Tan X et al. Fibroblast Growth Factor 10 Attenuates Renal Damage by Regulating Endoplasmic Reticulum Stress After Ischemia-Reperfusion Injury. Front Pharmacol 11:39 (2020). PubMed: 32116715
- Bell PA et al. Mesencephalic astrocyte-derived neurotropic factor is an important factor in chondrocyte ER homeostasis. Cell Stress Chaperones 24:159-173 (2019). PubMed: 30543055
- Piróg KA et al. XBP1 signalling is essential for alleviating mutant protein aggregation in ER-stress related skeletal disease. PLoS Genet 15:e1008215 (2019). PubMed: 31260448
- Bäuerlein FJB et al. In Situ Architecture and Cellular Interactions of PolyQ Inclusions. Cell 171:179-187.e10 (2017). PubMed: 28890085