Anti-PDIA6 抗体 (ab11432)
Key features and details
- Rabbit polyclonal to PDIA6
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-PDIA6 antibody
PDIA6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PDIA6 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Hamster, Xenopus laevis, Zebrafish
-
免疫原
Synthetic peptide corresponding to Human PDIA6 aa 476-492.
Sequence:DIDLSDVELDDLGKDEL
(Peptide available asab11432) -
ポジティブ･コントロール
- WB: A549, A431, HeLa, MCF7, Caco-2, HepG2, K-562 and COLO 205 cell lysates ICC/IF: A549 and HeLa cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11432の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/250.
|
|
| WB |
1/250. Detects a band of approximately 50 kDa.Can be blocked with Rabbit polyclonal to PDIA6 (ab11432).
|
| 特記事項 |
|---|
|
ICC/IF
1/250. |
|
WB
1/250. Detects a band of approximately 50 kDa.Can be blocked with Rabbit polyclonal to PDIA6 (ab11432). |
ターゲット情報
-
機能
May function as a chaperone that inhibits aggregation of misfolded proteins. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin. -
組織特異性
Expressed in platelets (at protein level). -
配列類似性
Belongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains. -
細胞内局在
Endoplasmic reticulum lumen. Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
-
参照データベース
- Entrez Gene: 10130 Human
- Entrez Gene: 286906 Rat
- Omim: 611099 Human
- SwissProt: Q15084 Human
- SwissProt: Q63081 Rat
- Unigene: 212102 Human
- Unigene: 580464 Human
- Unigene: 2685 Rat
-
別名
- Endoplasmic reticulum protein 5 antibody
- ER protein 5 antibody
- ERp5 antibody
see all
画像
-
Western blot - Anti-PDIA6 antibody (ab11432)All lanes : Anti-PDIA6 antibody (ab11432) at 1/250 dilution
Lane 1 : A549 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : MCF7 cell lysate
Lane 5 : K-562 cell lysate
Lane 6 : Caco-2 cell lysate
Lane 7 : HepG2 cell lysate
Lane 8 : COLO 205 cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L), HRP conjugate at 1/2500 dilution
Observed band size: 48 kDa why is the actual band size different from the predicted?Detected by chemiluminescence.
-
Immunocytochemistry/ Immunofluorescence - Anti-PDIA6 antibody (ab11432)Immunocytochemistry/ Immunofluorescence analysis of 70% confluent log phase A549 cells labeling PDIA6 with ab11432. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab11432 at 1/250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin, 1/300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification. -
Immunocytochemistry/ Immunofluorescence - Anti-PDIA6 antibody (ab11432)ICC/IF image of ab11432 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11432, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (13)
ab11432 は 13 報の論文で使用されています。
- Khalaf NB et al. Vascular endothelial ERp72 is involved in the inflammatory response in a rat model of skeletal muscle injury. Mol Med Rep 23:N/A (2021). PubMed: 33398381
- Cuddy LK & Mazzulli JR Analysis of lysosomal hydrolase trafficking and activity in human iPSC-derived neuronal models. STAR Protoc 2:100340 (2021). PubMed: 33659904
- Mao L et al. PDIA6 contributes to aerobic glycolysis and cancer progression in oral squamous cell carcinoma. World J Surg Oncol 19:88 (2021). PubMed: 33761940
- Yoneda A et al. Heat Shock Protein 47 Maintains Cancer Cell Growth by Inhibiting the Unfolded Protein Response Transducer IRE1a. Mol Cancer Res 18:847-858 (2020). PubMed: 32102897
- Lee HY et al. Phosphatidylinositol 3-kinase-d controls endoplasmic reticulum membrane fluidity and permeability in fungus-induced allergic inflammation in mice. Br J Pharmacol 177:1556-1567 (2020). PubMed: 31713846
