Anti-PDGFR alpha + Beta (phospho Y572 + Y574) 抗体 (ab5443)

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Western blot - Anti-PDGFR alpha + Beta (phospho Y572 + Y574) antibody (ab5443)

    Key features and details

    • Rabbit polyclonal to PDGFR alpha + Beta (phospho Y572 + Y574)
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-PDGFR alpha + Beta (phospho Y572 + Y574) antibody

    • 製品の詳細

      Rabbit polyclonal to PDGFR alpha + Beta (phospho Y572 + Y574)

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Mouse
      交差が予測される動物種: Rat, Human, Xenopus laevis

    • 免疫原

      Synthetic peptide corresponding to Human PDGFR alpha + Beta aa 572-574. This corresponds to Tyrosine 579 and 581 of Human PDGFR beta. The sequence is conserved in PDGFR alpha mouse, rat and frog.

    • ポジティブ･コントロール

      • NIH3T3 cells +/- PDGF.

    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab5443 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB Use a concentration of 0.35 - 1 µg/ml. Detects a band of approximately 170 - 185 kDa.

    ターゲット情報

    画像

    • Western blot - Anti-PDGFR alpha + Beta (phospho Y572 + Y574) antibody (ab5443)
      Western blot - Anti-PDGFR alpha + Beta (phospho Y572 + Y574) antibody (ab5443)
      Peptide Competition: Extracts prepared from NIH3T3 cells left unstimulated (1) and stimulated with PDGF (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/mL ab5443 antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab5443 blocks the antibody signal, thereby demonstrating the specificity of the antibody. Peptide Competition: Extracts prepared from NIH3T3 cells left unstimulated (1) and stimulated with PDGF

    参考文献 (4)

    ab5443 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab5443 は 4 報の論文で使用されています。

    • Mahadik K  et al. c-Abl-TWIST1 Epigenetically Dysregulate Inflammatory Responses during Mycobacterial Infection by Co-Regulating Bone Morphogenesis Protein and miR27a. Front Immunol 9:85 (2018). PubMed: 29449840
    • Ip CKM  et al. Neomorphic PDGFRA extracellular domain driver mutations are resistant to PDGFRA targeted therapies. Nat Commun 9:4583 (2018). PubMed: 30389923
    • Csak T  et al. MicroRNA-155 Deficiency Attenuates Liver Steatosis and Fibrosis without Reducing Inflammation in a Mouse Model of Steatohepatitis. PLoS One 10:e0129251 (2015). WB ; Mouse . PubMed: 26042593
    • Paugh BS  et al. Novel oncogenic PDGFRA mutations in pediatric high-grade gliomas. Cancer Res 73:6219-29 (2013). Human . PubMed: 23970477

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