Anti-PDGF BB 抗体 (ab23914)
Key features and details
- Rabbit polyclonal to PDGF B
- Suitable for: IHC-Fr, WB, IHC-P
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PDGF BB antibody
PDGF B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PDGF B -
由来種
Rabbit -
特異性
This antibody has been tested against PDGF B protein (ab9706) and has yet to be tested against endogenous protein.
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アプリケーション
適用あり: IHC-Fr, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human, Recombinant fragment
交差が予測される動物種: Rat, Cow
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab23914の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr | (1) |
Use at an assay dependent concentration.
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| WB | (4) |
Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14-18 kDa).
The predicted molecular weight of PDGF B is ~ 27 kDa according to the Swissprot entry P01127, however the reduced form of PDGF B runs between 14-18 kDa on a gel. |
| IHC-P | (4) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 特記事項 |
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IHC-Fr
Use at an assay dependent concentration. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14-18 kDa). The predicted molecular weight of PDGF B is ~ 27 kDa according to the Swissprot entry P01127, however the reduced form of PDGF B runs between 14-18 kDa on a gel. |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
- Information by UniProt
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参照データベース
- Entrez Gene: 540106 Cow
- Entrez Gene: 5155 Human
- Entrez Gene: 18591 Mouse
- Entrez Gene: 24628 Rat
- Omim: 190040 Human
- SwissProt: P01127 Human
- SwissProt: P31240 Mouse
- SwissProt: Q05028 Rat
see all -
別名
- Becaplermin antibody
- c sis antibody
- FLJ12858 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF B antibody (ab23914)This image is courtesy of an Abreview submitted by Lauren Carpenter
ab23914 staining PDGF B (green) in mouse skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 10 minutes at 25°C; antigen retrieval was by heat mediation in Tris pH 9. Samples were incubated with primary antibody (1/500 in antibody diluent reagent) for 14 hours at 4°C. An Alexa Fluor® 647-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. DAPI (blue) - nuclei.
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Western blot - Anti-PDGF B antibody (ab23914)All lanes : Anti-PDGF BB antibody (ab23914) at 1 µg/ml
Lane 1 :Recombinant human PDGF B protein (ab9706)
Lane 2 :Recombinant human PDGF B protein (ab9706) with Human PDGF B peptide (ab24035) at 1 µg
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 14-18 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?ab23914 detects a band of ~ 14 kDa corresponding to the reduced form of recombinant PDGF B (ab9706). The predicted molecular weight for PDGF B is 27 kDa according to the Swissprot entry, however the reduced form is known to run at between 14-18 kDa. The 14 kDa band is specifically blocked by the addition of the immunizing peptide, ab 24035.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF B antibody (ab23914)Image courtesy of Human Protein AtlasImage courtesy of Human Protein Atlas.
ab23914 staining PDGF B in Human kidney (male). The paraffin embedded tissue was incubated with ab23914 (1/500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab23914 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF B antibody (ab23914)This image is courtesy of an anonymous Abreviewab23914 staining PDGF B (green) in Mouse skeletal muscle (gastrocnemius) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with methacarn and blocked with 10% serum for 10 minutes at 25°C; antigen retrieval was by heat mediation in Tris pH 8. Samples were incubated with primary antibody (1/250 in antibody diluent reagent) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Red - WGA membranes, Blue - DAPI Nuclei.
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Western blot - Anti-PDGF B antibody (ab23914)Anti-PDGF BB antibody (ab23914) at 1 µg/ml + Recombinant Human PDGF B protein (ab73231) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14-18 kDa
Exposure time: 1 minute -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGF B antibody (ab23914)IHC image of PDGF B staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23914, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (38)
ab23914 は 38 報の論文で使用されています。
- Gao SY et al. PDGF-BB exhibited therapeutic effects on rat model of bisphosphonate-related osteonecrosis of the jaw by enhancing angiogenesis and osteogenesis. Bone 144:115117 (2021). PubMed: 31676407
- Ma Q et al. Osteoclast-derived apoptotic bodies couple bone resorption and formation in bone remodeling. Bone Res 9:5 (2021). PubMed: 33431863
- Tillie RJHA et al. A Switch from Cell-Associated to Soluble PDGF-B Protects against Atherosclerosis, despite Driving Extramedullary Hematopoiesis. Cells 10:N/A (2021). PubMed: 34359916
- Gao Y et al. Adipose-derived stem cells embedded in platelet-rich plasma scaffolds improve the texture of skin grafts in a rat full-thickness wound model. Burns 46:377-385 (2020). PubMed: 31852618
- Peng Y et al. Glucocorticoids Disrupt Skeletal Angiogenesis Through Transrepression of NF-?B-Mediated Preosteoclast Pdgfb Transcription in Young Mice. J Bone Miner Res 35:1188-1202 (2020). PubMed: 32078184