製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PDGF BB
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, ELISA, Neutralising, Sandwich ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Highly pure (>98%) recombinant hPDGF-BB (human Platelet Derived Growth Factor)

  • ポジティブ・コントロール
    • Recombinant human PDGF B protein (ab9706) can be used as a positive control in WB.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration. To detect hPDGF-BB by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hPDGF-BB.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hPDGF-BB (2.5 ng/ml), a concentration of 0.03 - 0.04 µg/ml of this antibody is required.
Sandwich ELISA Use at an assay dependent concentration. To be used with ab84265 as detection antibody and ab9706 as protein standard.
IHC-P Use a concentration of 1 µg/ml.

ターゲット情報

  • Information by UniProt
  • 参照データベース
  • 別名
    • Becaplermin antibody
    • c sis antibody
    • FLJ12858 antibody
    • Oncogene SIS antibody
    • PDGF 2 antibody
    • PDGF B chain antibody
    • PDGF Beta antibody
    • PDGF subunit B antibody
    • PDGF-2 antibody
    • PDGF2 antibody
    • Pdgfb antibody
    • PDGFB_HUMAN antibody
    • Platelet derived growth factor 2 antibody
    • Platelet derived growth factor B chain antibody
    • Platelet derived growth factor beta antibody
    • Platelet derived growth factor beta polypeptide (oncogene SIS) antibody
    • Platelet derived growth factor beta polypeptide (simian sarcoma viral (v sis) oncogene homolog) antibody
    • Platelet derived growth factor beta polypeptide antibody
    • Platelet-derived growth factor B chain antibody
    • Platelet-derived growth factor beta polypeptide antibody
    • Platelet-derived growth factor subunit B antibody
    • Proto-oncogene c-Sis antibody
    • Simian sarcoma viral (v sis) oncogene homolog antibody
    • SIS antibody
    • SSV antibody
    • v sis platelet derived growth factor beta polypeptide antibody
    see all

画像

  • ab9704 staining PDGF BB in normal human skin tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent proteinase K mediated antigen retrieval. The primary antibody was used at 1 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen.

  • Anti-PDGF B antibody (ab9704) at 0.2 µg/ml + A431 (Human) Whole Cell Lysate (ab52255) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Observed band size: 18,29 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 45 kDa (possible dimer)



    The 29kDa form is the propeptide.

参考文献

This product has been referenced in:
  • Zhao C  et al. Endothelial Cords Promote Tumor Initial Growth prior to Vascular Function through a Paracrine Mechanism. Sci Rep 6:19404 (2016). Read more (PubMed: 26762853) »
  • Yang J  et al. Platelet-derived growth factor mediates survival of leukemic large granular lymphocytes via an autocrine regulatory pathway. Blood 115:51-60 (2010). Neutralising ; Human . Read more (PubMed: 19880494) »
See all 3 Publications for this product

レビューと Q&A

1-5 of 5 Abreviews or Q&A

Question
Answer


The lab let me know that this antibody (ab84265, detection antibody) can be used with ab9704 as capture antibody and ab9706 as protein standard.

Read More

Answer

Thank you for contacting Abcam.
Earlier we discussed the specificity of ab9704 for detecting PDGF BB as well as the expected expression of PDGF BB across cell lines and tissues.
According to the human protein atlas, PDGF B (alternative name for PDGF BB), is seen across many different cell lines and in a variety of tissues. Specifically, it appears that lung macrophages have moderate - strong IHC staining for PDGF B in their cytoplasm. Below is a link to the protein atlas information:
http://www.proteinatlas.org/ENSG00000100311/normal
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Answer

Thank you for contacting us, I apologize for the delay in my reply. I would recommend ab114130 for your purposes. The immunogen for this antibody shows no significant similarity to PDGFA, and it is slightly less likely to react with mouse than our other rabbit polyclonal antibodies. That being said, the mouse protein is fairly homologous with human and there is still a chance that ab114130 may show some reactivity to mouse PGDF BB.
If you do want to use the mouse monoclonal antibody ab51869 on mouse tissue, you may need to perform an additional blocking step to prevent mouse-on-mouse background staining. If mouse-on-mouse background is a problem in your assay, it will be visible when running your no primary antibody or isotype control. To reduce this background, you can perform an additional blocking step after your normal serum block and before the primary antibody incubation. The sections should be incubated for 1 hour with a F(ab) fragment unconjugated secondary antibody to mouse IgG, such as ab6668, at a ˜1:100 dilution at room temperature.
I hope this helps, please let me know if you need any additional information or assistance.

Read More

Answer

I have pasted the supplier's protocol below, but please do not hesitate to contact us should you have any other questions. Neutralization Assay for Anti-h-PDGF-BB using 3T3 cells Antibody: Anti-Human PDGF Procedure: 1) Resuspend 3T3 cells in regular culture media with 10% calf serum (Gibco lot# 1023609). Transfer 100ml culture/well to assay plate (5,000 cells/well). Incubate overnight. 2) Complete media change. Replace with 100ml assay media containing 0.5% calf serum. Incubate overnight. 3) Complete media change with assay media. 4) Serial dilute a-h-PDGF in assay media in triplicate. 5) Transfer assay media containing 8ng/ml h-PDGF to serial diluted antibody. Volume/well is 120 ml. Incubate 1 hour. 6) Transfer 100ml h-PDGF/anti-h-PDGFcomplex to cells in assay plate. Volume of each well is 200ml,containing 2 ng/ml h-PDGF and anti-h-PDGFas indicated below. Incubate 46 hours. 7) Add 20ml of Promega Substrate Cell Titer 96 Aqueous One Solution Reagent to each well. 8) Incubate 37oC and read at OD 490 nm.

Read More

Question
Answer

Unfortunately, we do not have it in stock. However, from placing your order it will take approximately 2 working days for us to obtain this antibody, followed by another 24 hrs to ship the product to you.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録