Anti-PDE6D 抗体 (ab96825)
Key features and details
- Rabbit polyclonal to PDE6D
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PDE6D antibody
PDE6D 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PDE6D -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow, Dog, Zebrafish
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免疫原
Recombinant fragment corresponding to Human PDE6D aa 1-120.
Database link: NP_002592 -
ポジティブ･コントロール
- WB: MOLT4 whole cell lysate; mouse eye lysate. IHC-P: SAS xenograft tissue. ICC/IF: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96825の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/100 - 1/1000.
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| IHC-P |
1/100 - 1/1000.
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| WB |
1/500 - 1/5000. Predicted molecular weight: 17 kDa.
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| 特記事項 |
|---|
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ICC/IF
1/100 - 1/1000. |
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IHC-P
1/100 - 1/1000. |
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WB
1/500 - 1/5000. Predicted molecular weight: 17 kDa. |
ターゲット情報
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機能
Acts as a GTP specific dissociation inhibitor (GDI). Increases the affinity of ARL3 for GTP by several orders of magnitude and does so by decreasing the nucleotide dissociation rate. Stabilizes Arl3-GTP by decreasing the nucleotide dissociation. -
組織特異性
Retina. -
配列類似性
Belongs to the PDE6D/unc-119 family. - Information by UniProt
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参照データベース
- Entrez Gene: 281976 Cow
- Entrez Gene: 403781 Dog
- Entrez Gene: 5147 Human
- Entrez Gene: 18582 Mouse
- Entrez Gene: 363272 Rat
- Omim: 602676 Human
- SwissProt: Q95142 Cow
- SwissProt: Q9XT54 Dog
see all -
別名
- 5''-cyclic phosphodiesterase subunit delta antibody
- GMP PDE delta antibody
- GMP-PDE delta antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE6D antibody (ab96825)
Paraffin-embedded SAS xenograft tissue labeling PDE6D with ab96825 at 1/500 dilution in immunohistochemical analysis.
Antigen retrieval: EDTA-based (pH 8.0), 15 minutes.
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Immunocytochemistry/ Immunofluorescence - Anti-PDE6D antibody (ab96825)Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling PDE6D with ab96825 at 1/500 dilution (green) in ICC/IF.
The nuclear counter stain is Hoechst 33342 (blue).
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Western blot - Anti-PDE6D antibody (ab96825)Anti-PDE6D antibody (ab96825) at 1/5000 dilution + Mouse eye lysate at 50 µg
Secondary
Anti-Rabbit IgG HRP
Predicted band size: 17 kDa -
Western blot - Anti-PDE6D antibody (ab96825)Anti-PDE6D antibody (ab96825) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 17 kDa
12% SDS PAGE
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab96825 は 1 報の論文で使用されています。
- Chen YH et al. EPHA2 feedback activation limits the response to PDEd inhibition in KRAS-dependent cancer cells. Acta Pharmacol Sin N/A:N/A (2019). PubMed: 31316177