Anti-PDE6D 抗体 (ab5665)
Key features and details
- Rabbit polyclonal to PDE6D
- Suitable for: ICC/IF, WB, ICC, IHC-Fr
- Reacts with: Mouse, Sheep, Cow, Dog, Human, Pig
- Isotype: IgG
製品の概要
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製品名
Anti-PDE6D antibody
PDE6D 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PDE6D -
由来種
Rabbit -
特異性
This antibody does not detect other PDE6 isoforms. -
アプリケーション
適用あり: ICC/IF, WB, ICC, IHC-Frmore details -
種交差性
交差種: Mouse, Sheep, Cow, Dog, Human, Pig -
免疫原
Synthetic peptide corresponding to Mouse PDE6D aa 1-16.
Sequence:MSAKDERAR(D/E)ILRGFK
(Peptide available asab41788) -
ポジティブ･コントロール
- ICC/IF: B3 cells, BAEC cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5665の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/10 - 1/200.
|
|
| WB | (1) |
Use at an assay dependent concentration.
|
| ICC |
Use at an assay dependent concentration.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
Immunohistochemical staining of PDE6D in bovine retinal cross-sections using ab5665 results in staining of retinal outer segments. |
| 特記事項 |
|---|
|
ICC/IF
1/10 - 1/200. |
|
WB
Use at an assay dependent concentration. |
|
ICC
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. Immunohistochemical staining of PDE6D in bovine retinal cross-sections using ab5665 results in staining of retinal outer segments. |
ターゲット情報
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機能
Acts as a GTP specific dissociation inhibitor (GDI). Increases the affinity of ARL3 for GTP by several orders of magnitude and does so by decreasing the nucleotide dissociation rate. Stabilizes Arl3-GTP by decreasing the nucleotide dissociation. -
組織特異性
Retina. -
配列類似性
Belongs to the PDE6D/unc-119 family. - Information by UniProt
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参照データベース
- Entrez Gene: 281976 Cow
- Entrez Gene: 403781 Dog
- Entrez Gene: 5147 Human
- Entrez Gene: 18582 Mouse
- Omim: 602676 Human
- SwissProt: Q95142 Cow
- SwissProt: Q9XT54 Dog
- SwissProt: O43924 Human
see all -
別名
- 5''-cyclic phosphodiesterase subunit delta antibody
- GMP PDE delta antibody
- GMP-PDE delta antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-PDE6D antibody (ab5665)
Immunocytochemistry/Immunofluorescence analysis of PDE6D (green) showing staining in the cytoplasm and nucleus of B-3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5565 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunocytochemistry/ Immunofluorescence - Anti-PDE6D antibody (ab5665)Immunocytochemistry/Immunofluorescence analysis of PDE6D (green) showing staining in the cytoplasm and nucleus of BAEC cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5665 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab5665 は 2 報の論文で使用されています。
- Popow J et al. Highly Selective PTK2 Proteolysis Targeting Chimeras to Probe Focal Adhesion Kinase Scaffolding Functions. J Med Chem 62:2508-2520 (2019). PubMed: 30739444
- Li G et al. Generation of Retinal Organoids with Mature Rods and Cones from Urine-Derived Human Induced Pluripotent Stem Cells. Stem Cells Int 2018:4968658 (2018). PubMed: 30008752
