Anti-PDE6D 抗体 (ab5665)
Key features and details
- Rabbit polyclonal to PDE6D
- Suitable for: ICC/IF
- Reacts with: Mouse, Sheep, Cow, Human, Pig
- Isotype: IgG
製品の概要
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製品名
Anti-PDE6D antibody
PDE6D 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PDE6D -
由来種
Rabbit -
特異性
This antibody does not detect other PDE6 isoforms. -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Mouse, Sheep, Cow, Human, Pig -
免疫原
Synthetic peptide corresponding to Mouse PDE6D aa 1-16.
Sequence:MSAKDERAR(D/E)ILRGFK
(Peptide available asab41788) -
特記事項
This product was previously labelled as PDE6 delta
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab5665 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | 1/10 - 1/200. |
ターゲット情報
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機能
Acts as a GTP specific dissociation inhibitor (GDI). Increases the affinity of ARL3 for GTP by several orders of magnitude and does so by decreasing the nucleotide dissociation rate. Stabilizes Arl3-GTP by decreasing the nucleotide dissociation. -
組織特異性
Retina. -
配列類似性
Belongs to the PDE6D/unc-119 family. - Information by UniProt
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参照データベース
- Entrez Gene: 281976 Cow
- Entrez Gene: 5147 Human
- Entrez Gene: 18582 Mouse
- Omim: 602676 Human
- SwissProt: Q95142 Cow
- SwissProt: O43924 Human
- SwissProt: O55057 Mouse
- Unigene: 516808 Human
see all -
別名
- 5''-cyclic phosphodiesterase subunit delta antibody
- GMP PDE delta antibody
- GMP-PDE delta antibody
see all
画像
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Immunocytochemistry/Immunofluorescence analysis of PDE6D (green) showing staining in the cytoplasm and nucleus of B-3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5565 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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Immunocytochemistry/Immunofluorescence analysis of PDE6D (green) showing staining in the cytoplasm and nucleus of BAEC cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5665 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
プロトコール
データシートおよび資料
参考文献 (1)
ab5665 は 1 報の論文で使用されています。
- Li G et al. Generation of Retinal Organoids with Mature Rods and Cones from Urine-Derived Human Induced Pluripotent Stem Cells. Stem Cells Int 2018:4968658 (2018). PubMed: 30008752