Anti-PDE4D 抗体 (ab99409)
Key features and details
- Rabbit polyclonal to PDE4D
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PDE4D antibody
PDE4D 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PDE4D -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Corresponds to a region between amino acids 709-759 of Human PDE4D (NP_001098101.1).
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ポジティブ・コントロール
- HeLa whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99409の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 91 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 91 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. -
組織特異性
Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle. -
パスウェイ
Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1. -
関連疾患
Note=Genetic variations in PDE4D might be associated with susceptibility to stroke. PubMed:17006457 states that association with stroke has to be considered with caution. -
配列類似性
Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. -
翻訳後修飾
Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59. -
細胞内局在
Cytoplasm. Membrane. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome. - Information by UniProt
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参照データベース
- Entrez Gene: 5144 Human
- Omim: 600129 Human
- SwissProt: Q08499 Human
- Unigene: 117545 Human
- Unigene: 721404 Human
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別名
- cAMP specific 3',5' cyclic phosphodiesterase 4D antibody
- DKFZp686M11213 antibody
- 5''-cyclic phosphodiesterase 4D antibody
see all
画像
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All lanes : Anti-PDE4D antibody (ab99409) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 91 kDa
Exposure time: 30 seconds -
ab99409 at 1µg/ml detecting PDE4D in HeLa whole cell lysate by WB following IP.
Lane 1: ab99409 at 3µg/mg of lysate
Lane 2: IP with an antibody which recognizes an downstream epitope of PDE4D
Lane 3: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab99409 は論文での使用が確認できていません。