Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-PDE1C/PDE1 抗体 (ab14602)

Submit a review Q&A (2)References (6)
Western blot - Anti-PDE1C/PDE1 antibody (ab14602)

    Key features and details

    • Rabbit polyclonal to PDE1C/PDE1
    • Suitable for: IP, IHC-P, WB
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-PDE1C/PDE1 antibody
      PDE1C/PDE1 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to PDE1C/PDE1

    • 由来種

      Rabbit

    • 特異性

      The antibody does not cross react with PDE1A, PDE1B or other PDEs.

    • アプリケーション

      適用あり: IP, IHC-P, WBmore details

    • 種交差性

      交差種: Mouse, Rat, Human

    • 免疫原

      Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab14602の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IP
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration. PubMed: 19924104
    WB
    1/250 - 1/500. Predicted molecular weight: 70 kDa. This antibody labels multiple bands in the 70-87 kDa range all believed to be various PDE1C variants.
    特記事項
    IP
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration. PubMed: 19924104
    WB
    1/250 - 1/500. Predicted molecular weight: 70 kDa. This antibody labels multiple bands in the 70-87 kDa range all believed to be various PDE1C variants.

    ターゲット情報

    • 機能

      Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a high affinity for both cAMP and cGMP.

    • 組織特異性

      Expressed in several tissues, including brain and heart.

    • 配列類似性

      Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • 5''-cyclic nucleotide phosphodiesterase 1C antibody
      • Calcium/calmodulin dependent 3'5' cyclic nucleotide phosphodiesterase 1C antibody
      • Calcium/calmodulin-dependent 3'' antibody
      • Cam PDE 1C antibody
      • Cam-PDE 1C antibody
      • hCam 3 antibody
      • hCam-3 antibody
      • HCAM3 antibody
      • HSPDE1C1A antibody
      • Human 3'5' cyclic nucleotide Phosphodiesterase antibody
      • PDE 1C antibody
      • Pde1c antibody
      • PDE1C_HUMAN antibody
      • Phosphodiesterase 1C antibody
      • Phosphodiesterase 1C calmodulin dependent antibody
      • Phosphodiesterase 1C calmodulin dependent 70kDa antibody
      see all

    画像

    • Western blot - Anti-PDE1C/PDE1 antibody (ab14602)
      Western blot - Anti-PDE1C/PDE1 antibody (ab14602)
      All lanes : Anti-PDE1C/PDE1 antibody (ab14602) at 1/500 dilution

      Lane 1 : Brain lysate
      Lane 2 : Heart lysate
      Lane 3 : Lung lysate
      Lane 4 : Testis lysate
      Lane 5 : Kidney lysate

      Predicted band size: 70 kDa



      Western Blot analysis of PDE1C/PDE1 with ab14602 (1/500). Rat tissues: 1- Brain; 2 -Heart; 3 - Lung; 4 - Testis; 5 - Kidney.  

    参考文献 (6)

    ab14602 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab14602 は 6 報の論文で使用されています。

    • Li EA  et al. Phosphodiesterase expression in the normal and failing heart. Arch Biochem Biophys 662:160-168 (2019). PubMed: 30550727
    • Pinto CS  et al. Phosphodiesterase Isoform Regulation of Cell Proliferation and Fluid Secretion in Autosomal Dominant Polycystic Kidney Disease. J Am Soc Nephrol 27:1124-34 (2016). PubMed: 26289612
    • He J  et al. Postnatal experience modulates functional properties of mouse olfactory sensory neurons. Eur J Neurosci 36:2452-60 (2012). PubMed: 22703547
    • Giachini FR  et al. Decreased cGMP level contributes to increased contraction in arteries from hypertensive rats: role of phosphodiesterase 1. Hypertension 57:655-63 (2011). WB ; Rat . PubMed: 21282562
    • Lee AC  et al. Olfactory marker protein is critical for functional maturation of olfactory sensory neurons and development of mother preference. J Neurosci 31:2974-82 (2011). WB ; Mouse . PubMed: 21414919
    • Wang X  et al. Cyclic nucleotide signaling in polycystic kidney disease. Kidney Int 77:129-40 (2010). IHC-P ; Mouse, Rat . PubMed: 19924104

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE Mouse cerebellum whole extract PRIMARY ANTIBODY anti-PDE1C (ab14602), diluted in 5% nonfat milk in TBST at 1:250. Incubated over night at 4C with agitation, followed by 4 times wash in TBST (10 min. ea.) DETECTION METHOD When West pico substrate from Pierce was used, no band or background signal was observed. When West femto substrate from Pierce was used, faint multiple bands were observed, however those bands did not seem to be with right size for PDE1C (maybe some of them could be the real bands, but no specifically strong bands were seen). POSITIVE AND NEGATIVE CONTROLS USED No real positive controls were used. When whole brain extract was run instead of cerebellar extract, the same pattern was seen. The cerebellum is the part of the brain which expresses the highest level of PDE1C protein according to multiple reports published so far. ANTIBODY STORAGE CONDITIONS Stored at -80C as aliquotes and brougt back to room temperature just before use (no freeze-thaw cycle). SAMPLE PREPARATION 10mM Tris/HCl pH7.6, 0.33M sucrose, 1mM EDTA, 1mM sodium orthovanadate, 50mM NaF, 25mM pyrophosphate, 100nM calyculin A, 1x phosphatase inhibitor cocktail I (sigma), 1x protease inhibitor cocktail (sigma) Cerebellum was homogenized in 9 volumes of above buffer and adjusted to 1x SDS-PAGE sample buffer by the addition of 5x sample buffer. AMOUNT OF PROTEIN LOADED 20 to 30 micrograms per lane ELECTROPHORESIS/GEL CONDITIONS Reducing SDS-PAGE, 5-20% Gradient SDS-PAGE gel. TRANSFER AND BLOCKING CONDITIONS Transfered onto pre-wet PVDF membrane using typical transfer buffer. Prestained MW marker was transfered nicely. After transfer, the membrane was blocked with 5% nonfat milk diluted in TBST for 1h at room temperature. SECONDARY ANTIBODY goat anti-rabbitIg-HRP conjugate from DAKO, diluted at 1:2000 in 5% nonfat milk in TBST, incubated for 2h at room temperature, followed by 4 times wash in TBST (10 min. ea.) HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 4 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Prepared fresh samples every time. Tried 1:250, 1:500 and 1:1000 dilution of the primary antibody. Used more sensitive substrate (West femto) as mentioned above. Tried whole brain extract in addition to cerebellum samples. The membrane after probed with this antibody (and did not give a band) was re-probed with a different antibody (anti-PKC gamma,it worked beautifully). ADDITIONAL NOTES I have used several different antibodies from Abcam and all of them have worked greatly except for this one. I wish your team can give me a nice technical help to solve the problem with this one. Thanks in advance. Atsushi

    Read More
    Answer

    Thank you for your enquiry. I am sorry to hear you are having a problem with ab14602. I would like to suggest the following modifications to your protocol: Perhaps playing with the milk concentration in the primary antibody solution may help. Removing milk completely may give a lot of background, but if you decrease it to maybe 3%, you may get a better signal. Also, try using 3%BSA in PBS for the blocking step. I would also try running a no primary control definitely because that would tell you if the signal is just from the secondary non specific binding. There is an image on the data sheet and references listed that may help as well. If these tips fail to help you and you have purchased the antibody within the past 90 days, I would be happy to offer you a replacement or refund. Please let me know if this helps and do not hesitate to contact us for further advice.

    Read More

    Question

    On your PDE1c western blot data sheet there are multiple bands from different tissue origin lysates. As we are having problems with size of PDE1c can you tell us or mark these sizes of pde1c splice variants.

    Read More
    Answer

    Thank you for your enquiry. I have added a very useful paper to the datasheet for this antibody which will describe the different PEDC1 splice variants in mouse, and their tissue specificity. Yan C et al. The calmodulin-dependent phosphodiesterase gene PDE1C encodes several functionally different splice variants in a tissue-specific manner. J Biol Chem 271:25699-706 (1996). I have included a link to the datasheet at the bottom of this email, which will in turn give you the link to the paper. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com