Anti-PD-L1 抗体 [PDL1/2744] - BSA and Azide free (ab269684)
Key features and details
- Mouse monoclonal [PDL1/2744] to PD-L1 - BSA and Azide free
- Suitable for: WB, Protein Array
- Reacts with: Mouse, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-PD-L1 antibody [PDL1/2744] - BSA and Azide free
PD-L1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [PDL1/2744] to PD-L1 - BSA and Azide free -
由来種
Mouse -
Tested Applications & Species
Application Species WB MouseHuman -
免疫原
Recombinant fragment aa 39-191. The exact sequence is proprietary.
Database link: Q9NZQ7 -
ポジティブ・コントロール
- WB: HeLa, Raji, MDA231, MCF-7, NIH3T3 and Panc-1 cell lysates
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特記事項
ab269684 is the PBS only version of ab269674.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A/G purified -
特記事項(精製)
Purified from Bioreactor Concentrate -
ポリ/モノ
モノクローナル -
クローン名
PDL1/2744 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Positive Controls
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab269684 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
アプリケーション | Species |
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WB |
Mouse
Human
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アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 33 kDa.
Treat samples with PNGase F to confirm the specificity of bands if necessary. PMID: 31327656 The observed band size is not exactly as predicted MWs in WB due to the different forms and modifications of PD-L1. Hu Isoform I-3: 20-33 kDa (predicted) Ms: 32.8 kDa (predicted) Actual band size in WB is between 40-60 kDa. Positive Control: B-CPAP, MD-MB-231, U87-MG Negative Control: SW480 |
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Protein Array |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 33 kDa. Treat samples with PNGase F to confirm the specificity of bands if necessary. PMID: 31327656 The observed band size is not exactly as predicted MWs in WB due to the different forms and modifications of PD-L1. Hu Isoform I-3: 20-33 kDa (predicted) Ms: 32.8 kDa (predicted) Actual band size in WB is between 40-60 kDa. Positive Control: B-CPAP, MD-MB-231, U87-MG Negative Control: SW480 |
Protein Array
Use at an assay dependent concentration. |
ターゲット情報
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機能
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. -
組織特異性
Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. -
配列類似性
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Cell membrane and Endomembrane system. - Information by UniProt
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参照データベース
- Entrez Gene: 29126 Human
- Entrez Gene: 60533 Mouse
- Omim: 605402 Human
- SwissProt: Q9NZQ7 Human
- SwissProt: Q9EP73 Mouse
- Unigene: 521989 Human
- Unigene: 245363 Mouse
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別名
- B7 H antibody
- B7 H1 antibody
- B7 homolog 1 antibody
see all
画像
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All lanes : Anti-PD-L1 antibody [PDL1/2744] (ab269674) at 2 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : Raji cell lysate
Lane 3 : MDA231 cell lysate
Lane 4 : MCF-7 cell lysate
Lane 5 : NIH3T3 cell lysate
Lane 6 : Panc-1 cell lysate
Predicted band size: 33 kDaThis data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab269674).
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Protein Array containing more than 19,000 full-length human proteins using ab269674.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab269674).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab269684 は論文での使用が確認できていません。