Anti-PD-L1 抗体 [ABM4E54] (ab210931)
Key features and details
- Mouse monoclonal [ABM4E54] to PD-L1
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-PD-L1 antibody [ABM4E54]
PD-L1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [ABM4E54] to PD-L1 -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, WB, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human PD-L1 aa 1-250.
Database link: Q9NZQ7-1 -
ポジティブ・コントロール
- Recombinant protein fragment of Human PD-L1; Human Hodkin's lymphoma tissue. Programmed death-ligand 1 (PD-L1) expression in various tumor types - http://www.immunotherapyofcancer.org/content/1/S1/P53
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
ABM4E54 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab210931の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P | (1) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 33 kDa.
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Flow Cyt |
Use a concentration of 1 - 2 µg/ml.
|
特記事項 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 33 kDa. |
Flow Cyt
Use a concentration of 1 - 2 µg/ml. |
ターゲット情報
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機能
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. -
組織特異性
Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. -
配列類似性
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Cell membrane and Endomembrane system. - Information by UniProt
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参照データベース
- Entrez Gene: 29126 Human
- Omim: 605402 Human
- SwissProt: Q9NZQ7 Human
- Unigene: 521989 Human
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別名
- B7 H antibody
- B7 H1 antibody
- B7 homolog 1 antibody
see all
画像
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Immunohistochemical analysis of formalin-fixed paraffin-embedded Human Hodkin's lymphoma tissue, labeling PD-L1 with ab210931at 5 µg/mL.
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Anti-PD-L1 antibody [ABM4E54] (ab210931) at 0.5 µg/ml + Recombinant protein fragment of Human PD-L1
Predicted band size: 33 kDa -
Cell Surface flow cytometry analysis of PD-L1 in PHA treated human PBMC using 1 μg of ab210931. Green represents isotype control; pink represents ab210931 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.
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All lanes : Anti-PD-L1 antibody [ABM4E54] (ab210931) at 2 µg/ml
Lane 1 : A549 lysate
Lane 2 : MCF-7 lysate
Lane 3 : 293 lysate
Lane 4 : HCT-116 lysate
Lane 5 : Saos2 lysate
Lane 6 : HeLa lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 33 kDaExposure time: 1 minute in film and 30 seconds in imager.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (21)
ab210931 は 21 報の論文で使用されています。
- Park TI et al. Routine culture and study of adult human brain cells from neurosurgical specimens. Nat Protoc 17:190-221 (2022). PubMed: 35022619
- Li J et al. Gestational trophoblastic neoplasia with extrauterine metastasis but lacked uterine primary lesions: a single center experience and literature review. BMC Cancer 22:509 (2022). PubMed: 35524210
- Zhang Q et al. USP35 is a Potential Immunosuppressive Factor in Skin Cutaneous Melanoma. J Inflamm Res 15:3065-3082 (2022). PubMed: 35637872
- He HX et al. VISTA and PD-L1 synergistically predict poor prognosis in patients with extranodal natural killer/T-cell lymphoma. Oncoimmunology 10:1907059 (2021). PubMed: 33889438
- Ahn JH et al. A Novel Anti-PD-L1 Antibody Exhibits Antitumor Effects on Multiple Myeloma in Murine Models via Antibody-Dependent Cellular Cytotoxicity. Biomol Ther (Seoul) 29:166-174 (2021). PubMed: 33139584