製品の概要

  • 製品名
    Anti-PD-L1 antibody [ABM4E54]
    PD-L1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [ABM4E54] to PD-L1
  • 由来種
    Mouse
  • アプリケーション
    適用あり: IHC-P, WB, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment corresponding to Human PD-L1 aa 18-227.
    Sequence:

    AFTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQF VHGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMIS YGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIW TSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPE ENHTAELVIP


    Database link: Q9NZQ7-1

  • ポジティブ・コントロール
    • Recombinant protein fragment of Human PD-L1; Human Hodkin's lymphoma tissue. Programmed death-ligand 1 (PD-L1) expression in various tumor types - http://www.immunotherapyofcancer.org/content/1/S1/P53

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab210931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 33 kDa.
Flow Cyt Use a concentration of 1 - 2 µg/ml.

ターゲット情報

  • 機能
    Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
  • 組織特異性
    Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
  • 配列類似性
    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 細胞内局在
    Cell membrane and Endomembrane system.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B7 H antibody
    • B7 H1 antibody
    • B7 homolog 1 antibody
    • B7-H1 antibody
    • B7H antibody
    • B7H1 antibody
    • CD 274 antibody
    • CD274 antibody
    • CD274 antigen antibody
    • CD274 molecule antibody
    • MGC142294 antibody
    • MGC142296 antibody
    • OTTHUMP00000021029 antibody
    • PD L1 antibody
    • PD-L1 antibody
    • PD1L1_HUMAN antibody
    • PDCD1 ligand 1 antibody
    • PDCD1L1 antibody
    • PDCD1LG1 antibody
    • PDL 1 antibody
    • PDL1 antibody
    • Programmed cell death 1 ligand 1 antibody
    • Programmed death ligand 1 antibody
    • RGD1566211 antibody
    see all

画像

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Human Hodkin's lymphoma tissue, labeling PD-L1 with ab210931at 5 µg/mL.

  • Anti-PD-L1 antibody [ABM4E54] (ab210931) at 0.5 µg/ml + Recombinant protein fragment of Human PD-L1

    Predicted band size: 33 kDa

  • Cell Surface flow cytometry analysis of PD-L1 in PHA treated human PBMC using 1 μg of ab210931. Green represents isotype control; pink represents ab210931 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.

     

  • All lanes : Anti-PD-L1 antibody [ABM4E54] (ab210931) at 2 µg/ml

    Lane 1 : A549 lysate
    Lane 2 : MCF-7 lysate
    Lane 3 : 293 lysate
    Lane 4 : HCT-116 lysate
    Lane 5 : Saos2 lysate
    Lane 6 : HeLa lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 33 kDa



    Exposure time: 1 minute in film and 30 seconds in imager.

参考文献

This product has been referenced in:
  • Liu B  et al. PD-1/PD-L1 expression in a series of intracranial germinoma and its association with Foxp3+ and CD8+ infiltrating lymphocytes. PLoS One 13:e0194594 (2018). Read more (PubMed: 29617441) »
  • Qiu XY  et al. PD-L1 confers glioblastoma multiforme malignancy via Ras binding and Ras/Erk/EMT activation. Biochim Biophys Acta 1864:1754-1769 (2018). Read more (PubMed: 29510196) »
See all 4 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (SVV-infected Rhesus Lung Tissue)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
Yes - Triton X100
Specification
SVV-infected Rhesus Lung Tissue
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Colin Coleman

Verified customer

投稿 Aug 11 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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