Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U87MG glioblastoma)
Permeabilization
Yes - 0.1% v/v Triton X-100 pH 7.4, 5 min at RT
Specification
U87MG glioblastoma
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Diluent: PBS
Dilution
1/200
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: CF568 Donkey anti-rabbit IgG(H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Other
Host species: Donkey
Clonality: Polyclonal
Conjugation: Other
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Dimitra Kalamida
Verified customer
投稿 Sep 12 2017