Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (adult spinal cord DRG, T/S)
Specification
adult spinal cord DRG, T/S
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Other product details
Dilution
1/6000
Incubation time
2 minute(s) · Diluent: TBS/BSA/Azide
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti mouse Igs
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
Perfused-fixed rat spinal cord plus DRGs was dissected out and sampled to give 3 T/S slices of cervical, thoracic and lumbar regions. These three pieces were then processed to pwax (after further immersion-fixation for 3 days) and embedded in one pwax block (this minimises the number of pwax blocks).
Sections were cut at 5microns, floated out on a 48C water bath and mounted onto adhesive -coated slides and dried in a 62C oven for 2/24hrs.
My std stABCpx-DAB immunomethod.
Picture shows a DRG that has many positive nuclei.
To demonstrate only highly positive nuclei (those that are are cycling/departing cycling) one would need to further dilute this Ab reagent.
Sections were cut at 5microns, floated out on a 48C water bath and mounted onto adhesive -coated slides and dried in a 62C oven for 2/24hrs.
My std stABCpx-DAB immunomethod.
Picture shows a DRG that has many positive nuclei.
To demonstrate only highly positive nuclei (those that are are cycling/departing cycling) one would need to further dilute this Ab reagent.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 Nov 27 2007