Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Paget's disease of the nipple)
Specification
Paget's disease of the nipple
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1%
Other product details
Dilution
1/6000
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/Azide pH7.5
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti mouse Igs
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
I can only add that this anti PCNA clone ( PC10) is one of those antibody reagents that are remarkable in their consistency, specificity, avidity. The fact that it is a mouse monoclonal Ab yet gives such an extraordinary dilution factor is highly unusual.This anonymous pwax block is over 20 years old.
Caveat emptor: PCNA has a very long half-life, unlike Ki67: cells that are PCNA-positive are not neccessarily cycling cells.
Caveat emptor: PCNA has a very long half-life, unlike Ki67: cells that are PCNA-positive are not neccessarily cycling cells.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
投稿 Nov 19 2007