Anti-PCNA 抗体 [EPR3822] (ab92729)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3822] (ab92729)](https://www.abcam.co.jp/ps/products/92/ab92729/Images/ab92729-107938-anti-pcna-antibody-epr3822-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3822] (ab92729)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3822] to PCNA
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-PCNA antibody [EPR3822]
PCNA 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3822] to PCNA -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: ICC or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse
-
免疫原
Synthetic peptide within Human PCNA aa 200-300 (C terminal). The exact sequence is proprietary.
-
ポジティブ･コントロール
- HeLa, HepG2 and 293T cell lysates; Human colonic adenocarcinoma tissue; Human tonsil tissue
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR3822 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab92729の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | ||
| IHC-P |
| 特記事項 |
|---|
追加情報
Flow Cyt: 1/10.
IHC-P: 1/50 - 1/100. Antigen retrieval is recommended before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended.
WB: 1/1000 - 1/5000. Predicted molecular weight: 29 kDa.
Is unsuitable for ICC or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100. Antigen retrieval is recommended before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended.
WB: 1/1000 - 1/5000. Predicted molecular weight: 29 kDa.
Is unsuitable for ICC or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
-
機能
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. -
配列類似性
Belongs to the PCNA family. -
翻訳後修飾
Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation. -
細胞内局在
Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. - Information by UniProt
-
参照データベース
- Entrez Gene: 5111 Human
- Entrez Gene: 18538 Mouse
- Omim: 176740 Human
- SwissProt: P12004 Human
- SwissProt: P17918 Mouse
- Unigene: 147433 Human
- Unigene: 728886 Human
- Unigene: 7141 Mouse
-
別名
- ATLD2 antibody
- cb16 antibody
- Cyclin antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3822] (ab92729)
ab92729, at 1/50 dilution, staining PCNA in paraffin embedded Human colonic adenocarcinoma tissue by Immunohistochemisty.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3822] (ab92729)](https://www.abcam.co.jp/ps/products/92/ab92729/Images/ab92729-107937-anti-pcna-antibody-epr3822-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3822] (ab92729)ab92729, at 1/50 dilution, staining PCNA in paraffin embedded Human tonsil tissue by Immunohistochemisty.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Western blot - Anti-PCNA antibody [EPR3822] (ab92729)](https://www.abcam.co.jp/ps/products/92/ab92729/Images/ab92729-107939-anti-pcna-antibody-epr3822-western-blot.jpg)
Western blot - Anti-PCNA antibody [EPR3822] (ab92729)All lanes : Anti-PCNA antibody [EPR3822] (ab92729) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 29 kDa -
![Anti-PCNA antibody [EPR3822] (ab92729)](https://www.abcam.co.jp/ps/products/92/ab92729/Images/ab92729-4-benefits-of-recombinant-antibodies.png)
Anti-PCNA antibody [EPR3822] (ab92729)
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (6)
ab92729 は 6 報の論文で使用されています。
- Liu X et al. CREB1 acts via the miR-922/ARID2 axis to enhance malignant behavior of liver cancer cells. Oncol Rep 45:N/A (2021). PubMed: 33786634
- Williams TL et al. Making urinary extracellular vesicles a clinically tractable source of biomarkers for inherited tubulopathies using a small volume precipitation method: proof of concept. J Nephrol 33:383-386 (2020). PubMed: 31586298
- Tohmé R et al. Direct activation of PP2A for the treatment of tyrosine kinase inhibitor-resistant lung adenocarcinoma. JCI Insight 4:N/A (2019). PubMed: 30830869
- McClinch K et al. Small-Molecule Activators of Protein Phosphatase 2A for the Treatment of Castration-Resistant Prostate Cancer. Cancer Res 78:2065-2080 (2018). PubMed: 29358171
- Hamajima N et al. Increased Protein Stability of CDKN1C Causes a Gain-of-Function Phenotype in Patients with IMAGe Syndrome. PLoS One 8:e75137 (2013). WB ; Human . PubMed: 24098681
- Hanel ML et al. Facioscapulohumeral muscular dystrophy (FSHD) region gene 1 (FRG1) is a dynamic nuclear and sarcomeric protein. Differentiation 81:107-18 (2011). WB . PubMed: 20970242
