Anti-PCK2 抗体 [EPR14224] - C-terminal (ab187145)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14224] to PCK2 - C-terminal
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PCK2 antibody [EPR14224] - C-terminal
PCK2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR14224] to PCK2 - C-terminal -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- HepG2, MCF7, HeLa, mouse brain, mouse kidney, rat brain, rat kidney and rat spleen lysates; Human liver tissue; HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR14224 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187145の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/500.
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特記事項 |
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Flow Cyt (Intra)
1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa). |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/500. |
ターゲット情報
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機能
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. -
パスウェイ
Carbohydrate biosynthesis; gluconeogenesis. -
関連疾患
Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD) [MIM:261650]. A metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait. -
配列類似性
Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 5106 Human
- Entrez Gene: 74551 Mouse
- Entrez Gene: 361042 Rat
- Omim: 261650 Human
- SwissProt: Q16822 Human
- SwissProt: Q8BH04 Mouse
- Unigene: 75812 Human
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別名
- EC 4.1.1.32 antibody
- GTP mitochondrial precursor antibody
- HGNC:8725 antibody
see all
画像
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Anti-PCK2 antibody [EPR14224] - C-terminal (ab187145) at 1/10000 dilution + HepG2 lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 71 kDa -
All lanes : Anti-PCK2 antibody [EPR14224] - C-terminal (ab187145) at 1/1000 dilution
Lane 1 : MCF7 lysate
Lane 2 : Hela lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 71 kDa -
All lanes : Anti-PCK2 antibody [EPR14224] - C-terminal (ab187145) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat kidney lysate
Lane 6 : Rat spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 71 kDa -
Immunofluorescence analysis of 4% paraformaldehyde-fixed HeLa cells, labeling PCK2 (green) with ab187145 at 1/500 dilution. Alexa Fluor®488-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).
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Intracellular Flow Cytometry analysis of PCK2 expression in 2% paraformaldehyde-fixed HeLa cells using ab187145 at 1/50 dilution (red) and a rabbit IgG as negative control (green). A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody.
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Immunohistochemical analysis of paraffin-embedded Human liver tissue, labeling PCK2 with ab187145 at 1/500 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (6)
ab187145 は 6 報の論文で使用されています。
- Wang Z et al. Anti-diabetic effects of linarin from Chrysanthemi Indici Flos via AMPK activation. Chin Herb Med 14:97-103 (2022). PubMed: 36120128
- Bluemel G et al. PCK2 opposes mitochondrial respiration and maintains the redox balance in starved lung cancer cells. Free Radic Biol Med 176:34-45 (2021). PubMed: 34520823
- Smolle E et al. Distribution and prognostic significance of gluconeogenesis and glycolysis in lung cancer. Mol Oncol 14:2853-2867 (2020). PubMed: 32777161
- Braun F et al. The proteomic landscape of small urinary extracellular vesicles during kidney transplantation. J Extracell Vesicles 10:e12026 (2020). PubMed: 33304478
- Zhang H et al. Vitamin D receptor targets hepatocyte nuclear factor 4a and mediates protective effects of vitamin D in nonalcoholic fatty liver disease. J Biol Chem 295:3891-3905 (2020). PubMed: 32051143
- Chen SH et al. MicroRNA-351 eases insulin resistance and liver gluconeogenesis via the PI3K/AKT pathway by inhibiting FLOT2 in mice of gestational diabetes mellitus. J Cell Mol Med 23:5895-5906 (2019). PubMed: 31287224