Anti-PCID1 抗体 (ab237504)
Key features and details
- Rabbit polyclonal to PCID1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-PCID1 antibody
PCID1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PCID1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse, Chicken, Cow, Orangutan -
免疫原
Recombinant full length protein corresponding to Human PCID1 aa 1 to the C-terminus.
Database link: Q7L2H7 -
ポジティブ・コントロール
- WB: HeLa, HepG2 and A549 whole cell lysates; Rat heart and spleen lysates. IHC-P: Human testis and pancreatic cancer tissue. ICC/IF: HepG2 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purity greater than 95% -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab237504の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/20 - 1/200.
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WB |
1/500 - 1/5000. Predicted molecular weight: 43 kDa.
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ICC/IF |
1/50 - 1/200.
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特記事項 |
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IHC-P
1/20 - 1/200. |
WB
1/500 - 1/5000. Predicted molecular weight: 43 kDa. |
ICC/IF
1/50 - 1/200. |
ターゲット情報
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機能
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. May favor virus entry in case of infection with herpes simplex virus 1 (HSV1) or herpes simplex virus 2 (HSV2). -
組織特異性
Broadly expressed. -
配列類似性
Belongs to the eIF-3 subunit M family.
Contains 1 PCI domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 421602 Chicken
- Entrez Gene: 10480 Human
- Entrez Gene: 98221 Mouse
- Entrez Gene: 295975 Rat
- GenBank: BC019103 Human
- Omim: 609641 Human
- SwissProt: Q5ZJ64 Chicken
- SwissProt: Q3T148 Cow
see all -
別名
- B5 antibody
- B5 receptor antibody
- Dendritic cell protein antibody
see all
画像
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All lanes : Anti-PCID1 antibody (ab237504) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : Rat heart lysate
Lane 5 : Rat spleen lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 43 kDa -
Paraffin-embedded human testis tissue stained for PCID1 using ab237504 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human pancreatic cancer tissue stained for PCID1 using ab237504 at 1/100 dilution in immunohistochemical analysis.
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HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for PCID1 (green) using ab237504 at 1/100 dilution in ICC/IF. Secondary antibody is an Alexa-Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab237504 は論文での使用が確認できていません。